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Paper Information

Journal:   INTERNATIONAL JOURNAL OF HEALTH STUDIES   2016 , Volume 2 , Number 3; Page(s) 10 To 13.
 
Paper: 

Gene Expression Analysis of SOX2, NANOG, KLF4, OCT4, and REX1 Genes in Cord Blood Mononuclear Cells Treated with External Stresses

 
 
Author(s):  Sardarabadi Parvaneh, SOLEIMANI MASOUD*, ATASHI AMIR, POUR BEIRANVAND SHAHRAM, AKHAVAN RAHNAMA MAHSHID, ANBARLOU AZADEH
 
* Dept. of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
 
Abstract: 
Background: Induced pluripotent stem cells (iPSCS) can be obtained from autologous cells for therapeutic purposes. So, far, many studies have been done to produce induced pluripotent cells by transferring specific pluripotency genes using different methods. In this study, pluripotency gene expression induced by external stresses was assessed in cord blood mononuclear cells. Methods: In this experimental study, mononuclear cells were isolated from umbilical cord blood. Isolated cells were divided into three groups. The first group had been exposed to HCL (pH 5. 7) for 25 minutes and then transferred to the medium with normal pH. The second group was triturated with hamilton syringe for 15 min (external pressure), and the last group was considered the control group and did not receive treatment. Then, total RNA was extracted on Day 7. Gene expression of OCT4, SOX2, NANOG, REX1, and KLF4 was evaluated using qRT-PCR. Results: Gene expression of OCT4, NANOG, REX1, and KLF4 was increased after exposure to acidic pH and external pressures in comparison with control cells (P < 0. 05). SOX2 gene expression was decreased in cells exposed to acidic pH but increased by external pressure. Conclusions: Exposure of umbilical cord blood mononuclear cells to acidic pH and external pressure lead to re-activation of pluripotency genes in mature cells. These findings indicate that mature cells may be reprogrammed with manipulation of environmental conditions.
 
Keyword(s): Reprogramming,External pressure,Acidic pH,Mononuclear cells,Cord blood
 
 
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APA: Copy

Sardarabadi, P., & SOLEIMANI, M., & ATASHI, A., & POUR BEIRANVAND, S., & AKHAVAN RAHNAMA, M., & ANBARLOU, A. (2016). Gene Expression Analysis of SOX2, NANOG, KLF4, OCT4, and REX1 Genes in Cord Blood Mononuclear Cells Treated with External Stresses. INTERNATIONAL JOURNAL OF HEALTH STUDIES, 2(3), 10-13. https://www.sid.ir/en/journal/ViewPaper.aspx?id=666004



Vancouver: Copy

Sardarabadi Parvaneh, SOLEIMANI MASOUD, ATASHI AMIR, POUR BEIRANVAND SHAHRAM, AKHAVAN RAHNAMA MAHSHID, ANBARLOU AZADEH. Gene Expression Analysis of SOX2, NANOG, KLF4, OCT4, and REX1 Genes in Cord Blood Mononuclear Cells Treated with External Stresses. INTERNATIONAL JOURNAL OF HEALTH STUDIES. 2016 [cited 2021July25];2(3):10-13. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=666004



IEEE: Copy

Sardarabadi, P., SOLEIMANI, M., ATASHI, A., POUR BEIRANVAND, S., AKHAVAN RAHNAMA, M., ANBARLOU, A., 2016. Gene Expression Analysis of SOX2, NANOG, KLF4, OCT4, and REX1 Genes in Cord Blood Mononuclear Cells Treated with External Stresses. INTERNATIONAL JOURNAL OF HEALTH STUDIES, [online] 2(3), pp.10-13. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=666004.



 
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