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Paper Information

Journal:   IRANIAN JOURNAL OF WOOD AND PAPER SCIENCE RESEARCH   FALL 2017 , Volume 32 , Number 3 (60) ; Page(s) 422 To 435.

Laboratory study of synergistic effects of mixtures of organic biocides, metal chelator, and antioxidant materials against rainbow white-rot fungus

* Department of Wood Science and Paper Technology, Karaj Azad University, Karaj, Iran
In this research, the individual and interaction effects of three organic biocides (propiconazole, tebuconazole, and chlorothalonile) mixed the metal EDTA and the antioxidant BHT at different concentrations (50, 150, 250, 350, and 450 ppm) against the white-rot fungus, Trametes versicolor was investigated. The tests were done in the in vitro and using of sterilized malt extract agar in the wet autoclave. First, preservative solutions were prepared with different formulations and concentrations separately and then combined with water solvent. Under laminar sterile hood, about 25 mL of the media was poured into the Petri plates. Three antibiogram discs (with triangular position) were placed on the media. By micro-sampler, about 20 μ L of sterilized preservative solutions was poured on every disc, separately. Then, about 5 mm of mycelium plug of intended fungus that was inoculated previously was put in the center of plates. The plates were incubated at 23 ° C and 75% relative humidity and the mycelium growth of fungus and inhibition effects of different preservative solutions against fungus was measured daily for one week, . The results of this research demonstrated that chlorothalonil biocide by itself have not impose any inhibition effect on growth of fungus, whilst propiconazole and tebuconazole biocides by itself have significant effect at different concentrations on growth of fungus. In order to determined the synergistic effect of propiconazole, tebuconazole, and chlorothalonil biocides with non-biocides addition of EDTA and BHT, it has been found that there exist synergistic effect between propiconazole biocide and the EDTA at high concentration such as 450 ppm, but in combination with other solutions, synergistic effect has not been found. Also, no synergistic effect was found between tebuconazole and chlorothalonil biocides with other preservative solutions.
Keyword(s): Organic biocides,EDTA,BHT,growth inhibition,synergism,Trametes versicolor
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