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Paper Information

Journal:   INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)   FEBRUARY 2019 , Volume 17 , Number 2; Page(s) 127 To 134.
 
Paper: 

Protective effect of vitamin E on oxidative stress and sperm apoptosis in diabetic Mice

 
DOI: 

10.18502/ijrm.v17i2.3990

 
Author(s):  Mirzaei Khorramabadi Khadijeh, TALEBI ALI REZA*, ABBASI SARCHESHMEH ABOLGHASEM, Mirjalili Aghdas
 
* Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, Postal Code: 8916877391
 
Abstract: 
Background: Generation of free radicals and oxidative stress are a major contributor to diabetes. These factors lead to the development of diabetic testicles disorders. Objective: In this study, the protective effect of vitamin E on functional disorders associated with diabetes induced oxidative stress in male reproductive systems has been investigated. Materials and Methods: Thirty-three adult male Mice were divided into control, diabetic, and untreated diabetic groups. Streptozotocin was used to induce diabetes. In the treated group, vitamin E was given to the Mice intraperitoneally for 30 days. Then, animals were anesthetized and sacrificed. Animal testicles were isolated and homogenized in phosphate buffer and used for measuring sperm count, motility and survival of sperm, MDA concentration and antioxidant capacity (TAC). Apoptosis was also performed with the TUNEL test. Results: The results of reduction (12. 03± 98. 11) TAC, MDA concentration (– 28. 5± 2. 58), sperm motility (unstable sperma= 86. 4± 7. 48), sperm count (171. 51), Sperm morphology (natural morphology= 49. 69± 31. 93) and abnormal morphology (9. 77± 49. 7) with increased oxidative damage. These changes were statistically significant in comparison with the control group for all variables other than MDA (p= 0. 05). Treatment of vitamin E diabetic Mice improved the ability of antioxidants to prevent oxidative damage in the testicles, restore the sperm movement, and increase the number of normal sperm as well as TAC. The level of apoptosis in the treated group has decreased compared to the untreated group. Conclusion: Vitamin E protects the reproductive system against diabetes mellitus. Therefore, it was concluded that vitamin E may be a suitable agent for protecting the sperm and testicular parameters against undesirable effects of diabetes.
 
Keyword(s): Case-control study,Vitamin E,Diabetes treatment,Diabetic Syrian mice,Male reproductive dysfunction
 
References: 
 
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