Paper Information

Journal:   IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH   fall 2018-winter 2019 , Volume 26 , Number 2 (52) #a00483; Page(s) 177 To 195.
 
Paper: 

Analysis of genetic diversity of Pistacia atlantica Desf. populations from Zagros forests using ISSR, IRAP and SCoT molecular markers

 
 
Author(s):  Shah Ghobadi h., SHABANIAN N.*, KHADIVI A., RAHMANI M.S.
 
* Faculty of Natural Resources, University of Kurdistan, I.R.Iran
 
Abstract: 
This study was conducted to investigate intra-and inter-population genetic diversity of 15 populations of Pistacia atlantica from Zagros forests, represented by 181 genotypes, using 15, 12 and 13 primers based on three molecular marker including inter simple sequence repeats (ISSR), inter retrotransposal amplified polymorphism (IRAP) and start codon targeted (SCoT) markers, respectively. ISSR, IRAP and SCoT primers produced a total of 186, 143 and 136 bands, of which 162 (87. 0%), 130 (90. 9%) and 120 (88. 0%) showed polymorphisom, respectively. Results showed that all marker systems revealed relatively high genetic variation at population level (hISSR = 0. 22, PPLISSR = 61. 92%; hIRAP = 0. 25, PPLIRAP = 74. 19%; hSCoT = 0. 20, PPLSCoT = 64. 09%). According to ISSR, IRAP and SCoT based genetic differentiation coefficient (Φ ST; 0. 28, 0. 23, and 0. 22, respectively), a higher level of genetic variation was recorded within populations than that of among populations, that could be due to wind-based extensive gene flow among the populations. UPGMA cluster analysis based on three used molecular systems, devided the studied populations into distinct clusters. According to Mantel test, no significant correlation was recorded between genetic and geographic distances of the populations.
 
Keyword(s): Gene flow,IRAP,population genetics,SCoT,wild pistachio
 
References: 
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