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Paper Information

Journal:   IRANIAN JOURNAL OF WOOD AND PAPER SCIENCE RESEARCH   spring 2018 , Volume 33 , Number 1 (62) #l00556; Page(s) 88 To 99.
 
Paper: 

Extraction and identification of chemical compounds in acetone extract of Taxus baccata L bark

 
 
Author(s):  AFRAZ S., HOSSEINIHASHEMI S.K.*
 
* Department of Wood Science and Paper Technology, Karaj Azad University, Karaj, Iran
 
Abstract: 
In this research, the bark extract of the yew (Taxus baccata L. ) tree trunk growing in AfraTakhte forest cache of Aliabad-e Katul) located in Golestan province was extracted with acetone solvent and Soxhlet apparatus method and then the chemical components of the extract were identified, Three disks with thickness of 50 cm were cut from the breast height of the three trees felled due to the wind and/the landslide. The bark of disks was separated and milled to the wood flour. Then, extract soluble in acetone was prepared according to TAPPI standard method. For the silylation of compounds, some dry powder of extract was poured into a glass vial and the derivative materials (pyridine, BSTFA + TMCS 1%) were added. Then, the sample was injected to the gas chromatography-mass spectrometry device. The results from research indicated that the mean value of acetone extract of yew bark was 13. 63%. Generally, 46 compounds were identified in the yew bark. The most abundant identified compounds were: tetrasiloxane, decamethyl-(14. 80%), hexadecanoic acid, hydroxy ester (11. 24%), 3-(3-aminopropyl)-6-(3, 3-dimethylallylamino)purine (9. 85%), d-ribofuranose, 1, 2, 3, 5-tetrakis-O-(hydroxy)-(9. 31%), 2-methylpyrrolo[1, 2-a]pyrazin-1(2H)-one (6. 73%). The various chemical components such as phenolic, flavenoid, alkaloid, fatty acid, fatty acid ester, sugar, alkane, fatty alcohol, alcoholic sugar, and stroied were identified in the acetone extract of yew bark that some of them can synthesized as preservatives for inhibition of wood-degrading organisms. The most important identified cytotoxic and anticancer compounds in the acetone extract of yew bark has been 4-piperidinone, 2, 2, 6, 6-tetramethyl-(0. 19%) and phenol, 2, 4-bis(1, 1-dimethylethy)-(3. 32%).
 
Keyword(s): Yew bark,acetone extract,cytotoxic and anticancer components,4-Piperidinone,2,2,6,6-tetramethyl-
 
References: 
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