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Paper Information

Journal:   IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS   may-june 2017 , Volume 33 , Number 2 (82) #f00643; Page(s) 304 To 313.

Callus induction and phenolic acids production in apical meristem and embryo callus culture of Salvia leriifolia Benth

Author(s):  Modarres m.*
* Department of Biology, Farhangian University, Tehran, Iran
Salvia leriifolia Benth., belonging to Lamiaceae, has antinociceptive, anti-inflammatory, antidiabetic and antioxidant properties. In order to callus induction from apical meristem and embryo of salvia leriifolia to produce phenolic acids, an experiment was carried out in a completely randomized design. The apical meristem and embryo explants were cultured in MS medium supplemented with 2, 4-D (0, 1, 2, 3mg/L), KIN (0, 0. 3, 1mg/L), BAP (0, 0. 5, 1, 2, 3mg/L) and NAA (0, 0. 5, 1, 1. 5mg/L). The fresh and dry weights were evaluated and accumulation of phenolic acids was measured after four weeks of culture by HPLC. The best treatments for callus induction and growth was at the 2, 4-D 2mg/L and KIN 1mg/L. Accumulation of caffeic acid in embryo callus was higher than that of leaves of Salvia leriifolias and concentration of rosmarinic acid was comparable to the leaves. The highest salvianolic acid B concentration was found in callus of apical meristem that was about four times higher than that of leaves.
Keyword(s): Caffeic acid,rosmarinic acid,salvianolic acid B,callus
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