Paper Information

Journal:   IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS   may-june 2017 , Volume 33 , Number 2 (82) #f00643; Page(s) 244 To 255.

Optimizing the regeneration and rooting of Iranian native shallot (Allium stipitatum) via in vitro

Author(s):  Hajiheidar a., Tohidfar M., Miri S.M., Zarekarizi A.R.*, Ghadermazi S., Samiee Kh.
* Medicinal Plant Research Center, Institute of Medicinal Plants, Academic Center for Education Culture and Research, Karaj, Iran
This research was aimed to achieve the optimal protocol for micropropagation of shallot (Allium stipitatum) via basal disc. For regeneration, the basal disc explants were cultured on Murashig & Skoog (MS) medium containing different concentrations of Benzyladenine (BA) (0, 1 & 2 mgl-1) and Naphthalene acetic acid (NAA) (0, 0. 5 & 1 mgl-1). The regenerated bulblets were rooted on MS and ½ MS medium supplemented with different concentrations of BA, NAA, Indole-3-acetic acid (IAA) and activated charcoal (AC). The rooted plantlets were successfully acclimatized under ex vitro conditions. The results indicated that the maximum number of bulblet (12. 66) was observed on medium containing 2 mg. l-1 BA and 1 mg. l-1 NAA. In addition, the highest root number (1. 77) and length (2. 55 cm) were observed on 1 mg. l-1 IAA and 1 g. l-1 activated charcoal and the survival rate was 100% in pots filled with a coco peat: perlite: peat moss (1: 2: 1 v/v) mixture. This research could be a suitable method for micropropagation of this endangered plant. Hence, the rapid micropropagation of shallot is compulsory because of its various applications and extinction.
Keyword(s): Allium stipitatim,regeneration,rooting,activated charcoal
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