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Paper Information

Journal:   IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS   May-June 2018 , Volume 34 , Number 2 (88) ; Page(s) 272 To 285.

Morphological, phonological and molecular diversity in Iranian populations of Anthemis haussknechtii Boiss. & Reut.

* Seed and Plant Improvement Research Department, Hamedan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Hamedan, Iran
Molecular, morphological and phenological markers were used to detect genetic diversity in several populations of Anthemis haussknechtii' Boiss. & Reut. growing in six different provinces in center and west of Iran. Variance analysis of morphological and phenological traits showed that all traits were significantly different among populations. Essential oil percentage, number of flowering stems and flower diameter showed the highest coefficient of variation (45. 76, 38. 76 and 32. 86%, respectively). Six ISSR primers revealed 55 polymorphic bands, of which 38 (69. 1%) were polymorphic. The ranges of distance between populations were varied from 0. 009 to 0. 439. According to the derived dendrogram from UPGMA, at a similarity level of 0. 66, the populations were divided into two main groups. Grouping populations based on molecular markers was in accordance with geographical grouping. Among the populations, Ilam population is recommended for tropical areas with proper irrigation conditions, Ardakan and Khorramabad populations for commercial operators and research centers and Maybod population for cultivation in arid and semi-arid regions. Wide domain of genetic variation in this study could be considered as an available gene pool for A. haussknechtii improvement through selection and hybridization programs. Therefore, conservation strategies should be provided to maintain such diversity to apply in future breeding programs.
Keyword(s): Anthemis haussknechtii Boiss.& Reut.,selection,diversity,ISSR
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