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Paper Information

Journal:   IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS   January-February 2017 , Volume 32 , Number 6 (80) #P00532; Page(s) 958 To 967.

Callus induction and plant regeneration of Thymus daenensis Celak.

Author(s):  PIROOZ M., AMIRI H.*, Dostii B.
* Department of Biology, Lorestan University, Khoram Abad, Iran
Thymus daenensis Celak., belonging to the Lamiaceae family, is an endemic species of genus Thymus in Iran. Despite the increasing need for mass propagation of this plant due to the presence of useful secondary metabolites, there is little information about its proliferation. In the present study, the interaction of some hormonal treatments in the MS medium containing indole acetic acid (IAA), benzyl amino purine (BAP) and kinetin (Kin) in presence or absence ascorbic acid (AS) were investigated for callus induction and micropropagation of Thymus daenensis. Results showed that the callus index, fresh weight, and dry weight of callus from internode explants were better as compared with apical meristem explants. Significant differences were found for regeneration from callus cultures derived from apical meristem and internode, so that NAA-Kin (4. 5 and 1. 5 mg/L) and NAA-BAP (9 and 6 mg/L) were the best hormonal combination. As well, significant differences were found between callus production from internode explants and apical meristem explants, so that Kin-NAA (9 and 3 mg/L), and NAABAP (6 and 9 mg/L) in presence of ascorbic acid were the best hormonal combination. Overall, the control treatment, NAA and Kin showed favorable effects on callus induction and better regeneration was obtained from internode explants.
Keyword(s): Thymus daenensis Celak.,tissue culture,ascorbic acid,callus induction,regeneration
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