| Abstract:
In this research, the dependent and interaction effects of three organic pesticides (Propiconazole, Tebuconazole, and Chlorothalonile) and water-methanol extract of inner bark (endoderm) of barberry stem with different concentrations (50, 150, 250, 350, and 450 ppm) was investigated on the white-root fungus of Rainbow (Trametes versicolor). The tests were done in the in vitro and using malt-extract agar culture and agar-disk diffusion method (modified method of CLSI standard (2008)). The plates were placed inside the incubator at 23 ° C and 75% relative humidity for one week. In addition, the growth of mycelium fungus and inhibition effects of different preservative solutions against fungus was also measured daily for one week. The results demonstrated that inner bark extract of barberry stem and chlorothalonil pesticide did not alone have any inhibition effect on the growth of fungus, while the propiconazole and tebuconazole pesticides alone had a significant effect on the growth of fungus in various concentrations. In the study of the combined effects of preservative solutions showed that tebuconazole at the 450 ppm concentration and propiconazole at all of mentioned concentrations along with inner bark extract of barberry stem could prevent to the extent acceptable from the fungus growth. It can conclude that the propiconazole and tebuconazole pesticides along with the extract of the inner bark of barberry create a synergistic effect in preventing the growth of tested fungus.
|