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Paper Information

Journal:   MEDICAL JOURNAL OF THE ISLAMIC REPUBLIC OF IRAN (MJIRI)   2017 , Volume 31 , Number 1; Page(s) 0 To 0.
 
Paper: 

Antibody response to glycan antigens of hydatid cyst fluid, laminated layer and protoscolex of Echinococcus granulosus

 
 
Author(s):  Rafiei Sefiddashti Raheleh, SHARAFI SEYEDEH MARYAM, EBRAHIMI SOLTAN AHMAD, AKHLAGHI LAME, MOOSAVI ALI, ESKANDARIAN ABBASALI, HEJRATI ALIREZA, Yousofi Darani Hossein*
 
* Department of Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran
 
Abstract: 
Background: Hydatid disease is characterized by long-term growth of hydatid cysts in the human. The glycan antigens have an important role in the immunology of hydatid cyst. In this study immunological reaction of host sera to different glycan antigens of the cyst, has been investigated. Methods: The antibody responses were tested to glycoprotein and glycolipid of the laminated layer (LL), cyst fluid (CF) and protoscolex (PS) antigens of E. Granulosus using ELISA and western immunoblotting tests. Thin-layer chromatography and ß-elimination were used for glycan purification. Results: Both hydatid cyst and normal human sera reacted with hydatid cyst fluid, protoscolices, laminated layer, glycoprotein and glycolipid antigens. The most antigen-antibody reaction was related to CF and PS antigens, and LL antigens had the minimal reaction with the sera. Thin layer chromatography (TLC) of the antigens showed presence of many glycan bands in the laminated layer. Conclusion: The parasite may elaborate different glycan antigens in LL to evade host immune response.
 
Keyword(s): Echinococcus granulosus,Hydatid cyst,Laminated Layer,Thin-layer chromatography,Enzyme Linked Immunosorbent Assay,ß-elimination
 
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