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Paper Information

Journal:   PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL)   SPRING 2017 , Volume 15 , Number 1 ; Page(s) 36 To 42.
 
Paper: 

EVALUATING THE ABILITY OF BIOFILM FORMATION IN ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES IN ZAHEDAN

 
DOI: 

10.29252/jmj.15.1.36

 
Author(s):  JALILIAN SARA, ROKHBAKHSH ZAMIN FAROKH*
 
* DEPT OF MICROBIOLOGY, KERMAN BRANCH, ISLAMIC AZAD UNIVERSITY, KERMAN, IRAN
 
Abstract: 

Introduction: Escherichia coli (E.coli) is the main causative pathogen in urinary tract infections (UTIs). Antibiotic resistance based on Extended Spectrum -Lactamase (ESBL) producing Escherichia coli strains is reported to be the cause of community and hospital acquired infections therefore this this is an important problem in public health. The aim of this study was to identify the blaTEM, blaSHV and CTX genes in clinical isolates of E. coli recovered from patients with UTIs in Zahedan, Iran.
Methods and Materials: Escherichia coli isolates (N=60) were analyzed by biochemistry and phenotypic methods. Each of the initial E. coli screening test isolate was investigated for the presence of blaTEM, blaSHV and blaCTX-M genes via polymerase chain reaction (PCR) using gene-specific primers.
Results: Sixty tested isolates; the prevalence of blaTEM and blaCTX-M genes was determined to be 68.3%, 46.6% but blaSHV gene was not detected. Based on phenotypic tests, biofilm capacity was detected in 10 clinical isolates.
Conclusions: The results of this study show that the use of adequate doses of antibiotics in the treatment of infections may be caused by biofilms are emphasized. Rapid detection of biofilm formation, therefore isolates have the ability to decide care and management is necessary.

 
Keyword(s): ESCHERICHIA COLI, ANTIBIOTIC RESISTANCE, PCR, BIOFILM
 
 
References: 
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Citations: 
 
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APA: Copy

JALILIAN, S., & ROKHBAKHSH ZAMIN, F. (2017). EVALUATING THE ABILITY OF BIOFILM FORMATION IN ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES IN ZAHEDAN. PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL), 15(1 ), 36-42. https://www.sid.ir/en/journal/ViewPaper.aspx?id=573280



Vancouver: Copy

JALILIAN SARA, ROKHBAKHSH ZAMIN FAROKH. EVALUATING THE ABILITY OF BIOFILM FORMATION IN ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES IN ZAHEDAN. PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL). 2017 [cited 2021August04];15(1 ):36-42. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=573280



IEEE: Copy

JALILIAN, S., ROKHBAKHSH ZAMIN, F., 2017. EVALUATING THE ABILITY OF BIOFILM FORMATION IN ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES IN ZAHEDAN. PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL), [online] 15(1 ), pp.36-42. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=573280.



 
 
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