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Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   MAY 2018 , Volume 21 , Number 5; Page(s) 502 To 507.
 
Paper: 

ENDOPLASMIC RETICULUM STRESS REGULATES INFLAMMATION IN ADIPOCYTE OF OBESE RATS VIA TOLL-LIKE RECEPTORS 4 SIGNALING

 
 
Author(s):  LI XIAOHUA*
 
* DEPARTMENT OF ENDOCRINOLOGY, SEVENTH PEOPLE’S HOSPITAL OF SHANGHAI UNIVERSITY OF TCM, NO. 358, DATONG ROAD, GAOQIAO, PUDONG NEW AREA SHANGHAI, CHINA
 
Abstract: 

Objective (s): To explore whether endoplasmic reticulum (ER) stress regulates inflammation in adipose tissue of obese rats via TLR4 signaling.
Materials and Methods: Sprague Dawley rats were randomly divided into four groups, and body weight, food intake, and free fatty acids (FFA) were measured. Real-time PCR and Western blot were used to determine mRNA or protein expression of TLR4, TRAF6, IKK
b, TNF-a, IL-6, and GRP78. Immunohistochemistry was used to detect GRP78 protein expression.
Results: The FFA levels in HFD, HFD+PBA, and HFD+VIPER groups were higher than that in the control group (P<0.05). Compared with the control group, HFD induced GRP78 expression significantly (P<0.05), which could be decreased by ER stress inhibitor but not by TLR4 blocker. The mRNA expression of TLR4, TRAF6, TNF-
a, and IL-6, and protein levels of TLR4, TNF-a, and IKKb in the HFD group increased significantly compared with the control group (P<0.05), while these changes could be suppressed by PBA or VIPER (P<0.05). The immunohistochemistry staining indicated GRP78 expression in the HFD group was higher than that of the control group, which could be inhibited by PBA or VIPER.
Conclusion: HFD could induce inflammation in adipose tissue via ER stress and its downstream TLR4 signaling.

 
Keyword(s): ADIPOCYTE, ENDOPLASMIC RETICULUM STRESS, INFLAMMATION, OBESITY, TOLL-LIKE RECEPTOR 4
 
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