Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   MAY 2018 , Volume 21 , Number 5; Page(s) 465 To 468.
 
Paper: 

AN ANSWER TO COLON CANCER TREATMENT BY MESENCHYMAL STEM CELL ORIGINATED FROM ADIPOSE TISSUE

 
 
Author(s):  IPLIK ELIF SINEM, ERTUGRUL BARIS, KOZANOGLU ILKNUR, BARAN YUSUF, CAKMAKOGLU BEDIA*
 
* DEPARTMENT OF MOLECULAR MEDICINE, AZIZ SANCAR INSTITUTE FOR EXPERIMENTAL MEDICINE, ISTANBUL UNIVERSITY, ISTANBUL, TURKEY
 
Abstract: 

Objective (s): Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apoptotic effects of adipose tissue-derived MSCs in colon cancer.
Materials and Methods: MSCs were obtained from adipose tissue and characterized by Flowcytometer using suitable antibodies. MSCs, HT-29, HCT-116, RKO and healthy cell line MRC5 were cultured by different seeding procedure. After cell viability assay, changes in caspase 3 enzyme activity and the level of phosphatidylserine were measured.
Results: For cell viability assay, a 48 hr incubation period was chosen to seed all cells together. There was a 1.36-fold decrease in caspase 3 enzyme activity by co-treatment of RKO and MSCs in addition to 2.02-fold decrease in HT-29 and MSCs co-treatment, and 1.103-fold increase in HCT-116 and MSCs. The results demonstrated that HCT-116 led to the highest rate of apoptotic cell death (7.5%) compared with other cells.
Conclusion: We suggest that MSCs might remain a new treatment option for cancer by its differentiation and repair capacity.

 
Keyword(s): APOPTOSIS, COLON CANCER, STEM CELLS, CELL DEATH
 
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