Paper Information

Journal:   INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)   MARCH 2018 , Volume 16 , Number 3; Page(s) 183 To 190.
 
Paper: 

ALTERATION IN CATSPER1 AND 2 GENES EXPRESSION, SPERM PARAMETERS AND TESTIS HISTOLOGY IN VARICOCELIZED RATS

 
 
Author(s):  ZOHOUR SOLEIMANI MARYAM*, JALALI MASHAYEKHI FARIDEH, MOUSAVI HASANZADE MORTEZA, BAAZM MARYAM
 
* STUDENTS RESEARCH COMMITTEE, ARAK UNIVERSITY OF MEDICAL SCIENCES, ARAK, IRAN
 
Abstract: 

Background: CatSper gene, a member of cation channel sperm family, has an essential role in sperm motility and male fertility. Following varicocele, sperm parameters especially sperm movement decreases. For this reason, we hypothesized thatCatSper gene expression might be reduced after varicocele induction in an animal model.
Objective: The aim of this study was to evaluate the expression of CatSper 1 and 2 genes, sperm parameters and testis histology following varicocele induction.
Materials and Methods: A total of 30 Wistar male rats were randomly divided into three following groups (n=10/ each): control, sham, and varicocele group.
Experimental varicocele was induced by partial ligation of the left renal vein. The epididymal sperm parameters, CatSper 1 and 2 genes expression, and testes histology were studied two months after varicocele induction.
Results: Our results revealed that motility (32.73±16.14%), morphology (48.80±17%) and viability (31.23±9.82%) of sperms significantly reduced following varicocele induction. In addition, we showed a significant decrease in the number of spermatogonia (43.63±5.31) and seminiferous tubules diameters (190.51±19.23 mm) in experimental varicocele rats. The level ofCatSper 1 and 2 genes expression evaluated using real-time polymerase chain reaction was significantly downregulated 2 months after varicocele induction.
Conclusion: Our data indicated that experimental varicocele has deleterious effects on sperm parameters, testis structure as well as the expression ofCatSper 1 and 2 genes.

 
Keyword(s): VARICOCELE, CATSPER, SPERM PARAMETERS, RAT
 
References: 
 
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