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Paper Information

Journal:   JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES   SUMMER 2017 , Volume 19 , Number 2 (62) ; Page(s) 91 To 97.
 
Paper: 

DETECTION OF PHOSPHOLIPASE AND TYPE IV PILI GENES IN CLINICAL ISOLATES OF MULTIDRUG-RESISTANT PSEUDOMONAS AERUGINOSA

 
 
Author(s):  MONAZAMI A., HAGHI F.*
 
* DEPARTMENT OF MICROBIOLOGY AND VIROLOGY, ZANJAN UNIVERSITY OF MEDICAL SCIENCES, ZANJAN, IRAN
 
Abstract: 

Background and Objective: Pseudomonas aeruginosa is an opportunistic pathogen with numerous virulence factors such as phospholipase and type IV pili. The emergence of multidrug resistant Pseudomonas aeruginosahas become a serious public health threat worldwide. This study was done to determine the frequency of plcH, plcN, pilA and pilB genes in multi-drug resistantPseudomonas aeruginosaisolated from clinical samples.
Methods: In this cross-sectional study, 93 isolates of Pseudomonas aeruginosa collected from different clinical samples from hospitals of Zanjan, Iran during 2013-14. After identification of isolates by biochemical tests, antibiotic susceptibility testing (Kirby-Bauer) was performed according to CLSI guidelines. Total DNA extracted and PCR was done to detect of plcH, plcN, pilA and pilB genes.
Results: Among 93 of Pseudomonas aeruginosa isolates, the highest antibiotic resistance related to Erythromycin and Cefoxitin (95.6%) and the lowest resistance related to Amikacin (26.8%).80.6% of isolates were multidrug resistant (MDR). Out of 75 MDR isolates, the frequency of plcH, plcN, pilA and pilB genes was 97.4%, 49.3%, 26.6% and 17.3%, respectively.
Conclusion: According to high frequency of phospholipase C gene (plcH) in MDR Pseudomonas aeruginosaisolates which isolated from different clinical samples, presumably this virulence factor plays an important role in pathogenesis of this bacterium.

 
Keyword(s): EUDOMONAS AERUGINOSA, ANTIBIOTIC RESISTANCE, PHOSPHOLIPASE C, TYPE IV PILI
 
References: 
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