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Journal:   JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES   SUMMER 2017 , Volume 19 , Number 2 (62) ; Page(s) 91 To 97.
 
Paper: 

Detection Of Phospholipase And Type Iv Pili Genes In Clinical Isolates Of Multidrug-Resistant Pseudomonas Aeruginosa

 
 
Author(s):  Monazami A., Haghi F.*
 
* DEPARTMENT OF MICROBIOLOGY AND VIROLOGY, ZANJAN UNIVERSITY OF MEDICAL SCIENCES, ZANJAN, IRAN
 
Abstract: 
Background and Objective: Pseudomonas aeruginosa is an opportunistic pathogen with numerous virulence factors such as phospholipase and type IV pili. The emergence of multidrug resistant Pseudomonas aeruginosahas become a serious public health threat worldwide. This study was done to determine the frequency of plcH, plcN, pilA and pilB genes in multi-drug resistantPseudomonas aeruginosaisolated from clinical samples.Methods: In this cross-sectional study, 93 isolates of Pseudomonas aeruginosa collected from different clinical samples from hospitals of Zanjan, Iran during 2013-14. After identification of isolates by biochemical tests, antibiotic susceptibility testing (Kirby-Bauer) was performed according to CLSI guidelines. Total DNA extracted and PCR was done to detect of plcH, plcN, pilA and pilB genes.Results: Among 93 of Pseudomonas aeruginosa isolates, the highest antibiotic resistance related to Erythromycin and Cefoxitin (95.6%) and the lowest resistance related to Amikacin (26.8%).80.6% of isolates were multidrug resistant (MDR). Out of 75 MDR isolates, the frequency of plcH, plcN, pilA and pilB genes was 97.4%, 49.3%, 26.6% and 17.3%, respectively.Conclusion: According to high frequency of phospholipase C gene (plcH) in MDR Pseudomonas aeruginosaisolates which isolated from different clinical samples, presumably this virulence factor plays an important role in pathogenesis of this bacterium.
 
Keyword(s): EUDOMONAS AERUGINOSA, ANTIBIOTIC RESISTANCE, PHOSPHOLIPASE C, TYPE IV PILI
 
 
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APA: Copy

MONAZAMI, A., & HAGHI, F. (2017). DETECTION OF PHOSPHOLIPASE AND TYPE IV PILI GENES IN CLINICAL ISOLATES OF MULTIDRUG-RESISTANT PSEUDOMONAS AERUGINOSA. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, 19(2 (62) ), 91-97. https://www.sid.ir/en/journal/ViewPaper.aspx?id=572494



Vancouver: Copy

MONAZAMI A., HAGHI F.. DETECTION OF PHOSPHOLIPASE AND TYPE IV PILI GENES IN CLINICAL ISOLATES OF MULTIDRUG-RESISTANT PSEUDOMONAS AERUGINOSA. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES. 2017 [cited 2022May25];19(2 (62) ):91-97. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=572494



IEEE: Copy

MONAZAMI, A., HAGHI, F., 2017. DETECTION OF PHOSPHOLIPASE AND TYPE IV PILI GENES IN CLINICAL ISOLATES OF MULTIDRUG-RESISTANT PSEUDOMONAS AERUGINOSA. JOURNAL OF GORGAN UNIVERSITY OF MEDICAL SCIENCES, [online] 19(2 (62) ), pp.91-97. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=572494.



 
 
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