Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   MARCH 2018 , Volume 21 , Number 3; Page(s) 325 To 332.
 
Paper: 

BAX/BCL-2 EXPRESSION RATIO IN PREDICTION OF RESPONSE TO BREAST CANCER RADIOTHERAPY

 
 
Author(s):  AZIMIAN HOSEIN, DAYYANI MAHDIEH, BAHREYNI TOOSSI MOHAMMAD TAGHI*, MAHMOUDI MAHMOUD
 
* MEDICAL PHYSICS RESEARCH CENTER, MASHHAD UNIVERSITY OF MEDICAL SCIENCES, MASHHAD, IRAN
 
Abstract: 

Objective (s): Radiotherapy is one of the most effective modalities of cancer therapy, but clinical responses of individual patients varies considerably. To enhance treatment efficiency it is essential to implement an individual-based treatment. The aim of present study was to identify the mechanism of intrinsic apoptosis pathway on radio sensitivity and normal tissue complications caused by the radiotherapy.
Materials and Methods: Peripheral blood mononuclear cells from ten breast cancer patients were exposed to 6MV X-rays to deliver 1 and 2 Gy. Expression levels of Bax, Bcl-2, and Bax/Bcl-2 ratio were examined by relative quantitative RT-PCR. All the patients received similar tangential irradiation of the whole breast and conventional fractionation. Skin dosimetry was done by GAF Chromic EBT-3 film and clinical radio sensitivity was determined using the acute reactions to radiotherapy of the skin according to Radiation Therapy Oncology Group score. All statistical analyses were performed using Graph Pad Prism, version 7.01.
Results: In the in-vitro experiment, Bax and Bax/Bcl-2 ratios were significantly increased with 1 and 2 Gy doses (P<0.001 and P<0.0001, respectively). Herein, the notable result was a significant correlation between dose-response curve slope (as an in-vitro radio sensitivity index) and acute skin toxicity score following irradiation (as a clinical radio sensitivity index). There was no significant relationship between skin dose and reactions (P>0.05 for all patients).
Conclusion: Significant correlation between Bax/Bcl-2 ratio determined before radiation therapy and clinical response in the patients, can be used as a biomarker to identify radiosensitive individuals. However, further studies are required to validate radiation-induced apoptotic biomarkers.

 
Keyword(s): BAX, BCL-2, BREAST CANCER, RADIOTHERAPY, RADIOSENSITIVITY
 
References: 
  • ندارد
 
  pdf-File tarjomyar Yearly Visit 30
 
Latest on Blog
Enter SID Blog