Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   MARCH 2018 , Volume 21 , Number 3; Page(s) 253 To 259.
 
Paper:  THE CUCURBITACINS D, E, AND I FROM ECBALLIUM ELATERIUM (L.) UPREGULATE THE LC3 GENE AND INDUCE CELL-CYCLE ARREST IN HUMAN GASTRIC CANCER CELL LINE AGS
 
Author(s):  JAFARGHOLIZADEH NASER, ZARGAR SEYED JALAL*, AFTABI YOUNES
 
* DEPARTMENT OF CELL & MOLECULAR BIOLOGY, SCHOOL OF BIOLOGY, COLLEGE OF SCIENCE, UNIVERSITY OF TEHRAN, TEHRAN, IRAN
 
Abstract: 

Objective (s): Cucurbitacins exhibit a range of anti-cancer functions. We investigated the effects of cucurbitacins D, E, and I purified from Ecballium elaterium (L.) A. Rich fruits on some apoptotic and autophagy genes in human gastric cancer cell line AGS.
Materials and Methods: Using quantitative reverse transcription PCR (qRT-PCR), the expression of LC3, VEGF, BAX, caspase-3, and c-MYC genes were quantified in AGS cells 24 hr after treatment with cucurbitacins D, E, and I at concentrations 0.3, 0.1 and 0.5
mg/ml, respectively. Cell cycle and death were analyzed by flowcytometry.
Results: Purified cucurbitacins induced sub-G1 cell-cycle arrest and cell death in AGS cells and upregulated LC3 mRNA effectively, but showed a very low effect on BAX, caspase-3, and c-MYC mRNA levels. Also after treatment with cucurbitacin I at concentration 0.5
mg/ml, VEGF mRNA levels were increased about 4.4 times. Pairwise comparison of the effect of cucurbitacins D, E, and I on LC3 mRNA expression showed that the cucurbitacin I effect is 1.3 and 1.1 times that of cucurbitacins E and D, respectively; cucurbitacin D effect is 1.2 times that of cucurbitacin E (P-value <0.05). In silico analysis showed that among autophagy genes, LC3 has an important gastric cancer rank relation.
Conclusion: Cucurbitacins D, E, and I purified from E. elaterium fruits upregulate LC3 and induce sub-G1 cell-cycle arrest and cell death in human gastric cancer cell line AGS. Cucurbitacin I effect on LC3 mRNA expression is significantly more than that of cucurbitacins E and D.

 
Keyword(s): APOPTOSIS, AUTOPHAGY CELL CYCLE, CUCURBITACINS STOMACH NEOPLASMS
 
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