Paper Information

Journal:   IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES   MARCH 2018 , Volume 21 , Number 3; Page(s) 230 To 235.
 
Paper: 

AMELIORATION OF CARBON TETRACHLORIDE-INDUCED HEPATIC INJURY BY EMULSIFIED ANTRODIA EXTRACT

 
 
Author(s):  CHANG WEI CHIH, HUNG CHANG TSEN, CHEN YUH SHUEN, HSUEH CHIH CHI, HOU CHIEN WEI*, LAY HORNG LIANG
 
* DEPARTMENT OF BIOTECHNOLOGY AND PHARMACEUTICAL TECHNOLOGY, YUANPEI UNIVERSITY OF MEDICAL TECHNOLOGY, HSINCHU, TAIWAN
 
Abstract: 

Objective (s): Antrodia cinnamomea (AC) is found with anti-inflammatory and immunomodulatory biological activities. In this study, we investigated the anti-hepatitis effect of the emulsified AC extract from RO water or supercritical fluid CO2 with ethanol co-solvent extract methods of AC preparations.
Materials and Methods: Five groups of eight to ten weeks male rats with a count of ten for each group were studied to evaluate the protection of two kinds of AC extract from hepatic injury. Acute liver injury of rats was induced by injecting 40% carbon tetrachloride (CCl4) 1 mg/kg intraperitoneally. Positive and negative control groups rats were perfused with CCl4 or isotonic saline, respectively. Experimental groups received oral administration once/day of AC preparations before CCl4 treatment: water AC extract (WAE group), or emulsified AC extract from supercritical fluid extraction (EAE group) for 5 days, and sacrificed on the 6th day and the blood and liver samples were collected under chloral hydrate anesthesia. The anti-inflammatory, antioxidant markers, and relevant signaling pathways were measured (AST, ALT, ROS, IL-1, IL-6, NO, and COX-2, MAPKs, and caspase-3).
Results: EAE at 50 mg/kg significantly decreased the serum AST, ALT, IL-1, IL-6, NO, and ROS levels. Both extracts reduced the activation of p-ERK in the liver samples, but EAE inhibited COX-2 and caspase-3 protein expression better than WAE. The EAE ameliorated CCl4-induced hepatic injury significantly; as compared with WAE and the positive control.
Conclusion: The hepatoprotection of EAE could be attributed to the antioxidant and anti-inflammatory effects of Antrodia.

 
Keyword(s): ANTIOXIDANT, ANTI-INFLAMMATORY, ANTRODIA CINNAMOMEA, CARBON TETRACHLORIDE, HEPATOPROTECTION, MAPKS
 
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