Paper Information

Journal:   IRANIAN JOURNAL OF PEDIATRIC HEMATOLOGY AND ONCOLOGY   2018 , Volume 8 , Number 2 #M0084; Page(s) 75 To 86.
 
Paper: 

STUDY OF EPSTEIN-BARR VIRUS NUCLEAR ANTIGEN (EBNA-1) VARIATIONS: VVAL TYPE PREFERENTIALLY EXISTS IN BIOPSIES OF NASOPHARYNGEAL CARCINOMA FROM VIETNAMESE PATIENTS

 
 
Author(s):  LAO THUAN DUC, TRUONG PHUONG KIM, HUYEN LE THUY AI*
 
* FACULTY OF BIOTECHNOLOGY, HO CHI MINH CITY OPEN UNIVERSITY, VIETNAM
 
Abstract: 

Background: Epstein-barr virus nuclear antigen 1 (EBNA1) plays a crucial role in Nasopharyngeal carcinoma (NPC), the most common cancer of head and neck cancer in Asian countries with high incidents. Sequence variations are of high frequency within the functionally important domains ofEBNA-1, which have been classified into five subtypes: Phenotype (P) -ala, P-thr, V-val, V-leu and V-pro and are related to geographical location. This study aimed to evaluate the variations ofEBNA-1 in NPC biopsy samples from Vietnamese patients.
Materials and Methods: In this descriptive analytical study, 20 NPC biopsy samples, which were positively confirmed to NPC, were collected from Cho Ray Hospital. Nested PCR – nucleotide sequencing was applied to analyze the carboxy-terminal region ofEBNA-1. Phylogenetic analysis was performed using the Molecular Evolutionary Genetics Analysis, version 5.0, by Neighbor-Joining algorithm.
Results: The variants of EBNA-1 have been described based on the amino acid signature at codon 487, including V-val (75.0%), P-ala (10%), P-thr (5%), V-leu (5%). Additionally, the phylogenetic results confirmed that the V-val subtype, detected in this current study of the Vietnamese population, was in accordance with previous studies that V-val is almost an exclusive variation in Asian region, especially with Chinese populations and preferentially exists in biopsies of nasopharyngeal carcinoma.
Conclusion: In this initial study, the sub-strains of EBV within V-val subtype of EBNA-1 was preferential in biopsies of NPC patients. The finding provided the initial data for the potential contribution ofEBNA-1 polymorphisms to etiology of NPC in Vietnamese population.

 
Keyword(s): ESPTEIN-BARR VIRUS, NASOPHARYNGEAL CARCINOMA, NUCLEAR ANTIGEN, VARIATIONS
 
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