In order to investigate effects of temperature and light on germination of three Artemisia species, a factorial experiment with four replications was conducted in laboratory of Science and Research Branch- Islamic Azad University in 2013. Factors were three species of Artemisia (annual, biennial and perennial), temperature at nine levels (5, 10, 15, 20, 25, 30, 35, 40 and 45oC) and light regime at three levels (dark, 14 light/10 dark and light). Results showed that maximum germination and seedling growth of perennial Artemisia was at 30oC while it was 25 °C for two other species. Less the amount of studied characteristics such as germination percentage, radicle length, plumule length and seedling fresh in perennial Artemisia, possibly due to less investment of perennial weeds in seed production. Among investigated traits, germination percentage significantly influenced by species × light interaction. Highest percentage of germination (66.75) was seen at 25 °C and continuous light. This treatment is significantly different from other treatments. Knowledge about seed germination controlling factors will have an important role in weeds control.