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Paper Information

Journal:   JOURNAL OF CROP BIOTECHNOLOGY   FALL 2017 , Volume 7 , Number 19 ; Page(s) 1 To 13.
 
Paper: 

CLONING AND EXPRESSION OF AN OMIGANAN PENTAHYDROCHLORIDE (MBI226) ANTIMICROBIAL PEPTIDE IN TOBACCO (NICOTIANA TABACUM) CONFERS RESIST TO BACTERIAL PATHOGENS

 
 
Author(s):  BADRHADAD AZAM, NAZARIAN FIROUZABADI FARHAD*, ISMAILI AHMAD, BAGHERI HEDAYAT
 
* DEPARTMENT OF AGRONOMY AND PLANT BREEDING, FACULTY OF AGRICULTURE, LORESTAN UNIVERSITY, KHORRAMABAD, IRAN
 
Abstract: 

Antimicrobial peptides are ancient and conserved molecules which are found in defense mechanisms of almost all living organisms from bacteria to animal and plant species. Identification and introduction of novel antimicrobial peptides, is a cost-effective way to improve crop plants resistance to pathogens by using recombinant DNA technology. Therefore, an expression construct containing omiganan antimicrobial encoding gene from the cytoplasmic granules of bovine neutrophils, was cloned and transferred to the tobacco leaf disk using Agrobacterium tumefaciensmediated-transformation. The presence of the antimicrobial peptide encoding gene in the genome of transgenic plants was confirmed by PCR analysis. Six putative transgenic lines and a non-transgenic control plant were selected for further molecular analysis. Total protein was extract from transgenic and nontransgenic control plants and used for antimicrobial activity assay against some human; E. coli, Salmonella, Staphylococcus, Bacillus, and plant: Xanthomonas and Pseudomonaspathogens by disc diffusion method. Results of this experiment showed that total protein extract from transgenic lines, as compared to non-transgenic plant, was significantly (P<0.05) able to inhibit bacteria growth. There was a significant difference (P<0.05) between transgenic lines with respect to anti-bacteria effect. In contrary to gram-negative bacteria, omiganan peptide did not have a significant effect (P>0.05) on gram-positive pathogenic bacteria. Therefore, it is possible to express and purify omiganan peptide from transgenic and used it in pharmacology formulations as an anti-bacterial medicine. Furthermore, this approach may offer an opportunity to generate transgenic crop plants expressing omiganan peptide resistance to some devastating plant bacteria pathogens.

 
Keyword(s): ANTIMICROBIAL PEPTIDES, AGROBACTERIUM TUMEFACIENS, PATHOGEN, RECOMBINANT PROTEIN, TOBACCO
 
 
References: 
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Citations: 
 
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APA: Copy

BADRHADAD, A., & NAZARIAN FIROUZABADI, F., & ISMAILI, A., & BAGHERI, H. (2017). CLONING AND EXPRESSION OF AN OMIGANAN PENTAHYDROCHLORIDE (MBI226) ANTIMICROBIAL PEPTIDE IN TOBACCO (NICOTIANA TABACUM) CONFERS RESIST TO BACTERIAL PATHOGENS. JOURNAL OF CROP BIOTECHNOLOGY, 7(19 ), 1-13. https://www.sid.ir/en/journal/ViewPaper.aspx?id=571875



Vancouver: Copy

BADRHADAD AZAM, NAZARIAN FIROUZABADI FARHAD, ISMAILI AHMAD, BAGHERI HEDAYAT. CLONING AND EXPRESSION OF AN OMIGANAN PENTAHYDROCHLORIDE (MBI226) ANTIMICROBIAL PEPTIDE IN TOBACCO (NICOTIANA TABACUM) CONFERS RESIST TO BACTERIAL PATHOGENS. JOURNAL OF CROP BIOTECHNOLOGY. 2017 [cited 2021July24];7(19 ):1-13. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=571875



IEEE: Copy

BADRHADAD, A., NAZARIAN FIROUZABADI, F., ISMAILI, A., BAGHERI, H., 2017. CLONING AND EXPRESSION OF AN OMIGANAN PENTAHYDROCHLORIDE (MBI226) ANTIMICROBIAL PEPTIDE IN TOBACCO (NICOTIANA TABACUM) CONFERS RESIST TO BACTERIAL PATHOGENS. JOURNAL OF CROP BIOTECHNOLOGY, [online] 7(19 ), pp.1-13. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=571875.



 
 
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