Paper Information

Journal:   JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY)   WINTER 2017 , Volume 30 , Number 4 #P0095; Page(s) 694 To 700.
 
Paper: 

FLOW CYTOMETRIC DNA C-VALUE AND PLOIDY VARIATION IN SOME IRANIAN APPLE CULTIVARS

 
 
Author(s):  FARAMARZI SH.*, YADOLLAHI A., KARIMZADEH GH.
 
* HORTICULTURAL SCIENCE DEPARTMENT, UNIVERSITY OF HORMOZGAN
 
Abstract: 

Introduction: Apple (Malus Miller) belongs to Rosacease family and the subfamily of Pomoideae. This fruit is at first place among fruits of temperate zones. The cultivated apple (Malus´domestica Borkh.) is a complex hybrid of the apple species. Chromosomal basis of this subfamily are x=17 and the ploidy levels have been reported for diploid (2n=2x=34), triploid (2n=3x=51) and tetraploid (2n=4x=68). Since Iran is close to the apple diversity region (Central Asia), it has a good variation of apple varieties. Despite the high levels of variation in apple cultivars and species in Iran, there is not still a database of genome size. Classification of plants according to their genome size, especially at lower taxonomic levels is important for breeders. Over the past years, several methods for estimation of nuclear DNA content (genome size) was common, but recently, the use of flow cytometry (FCM) has been increasingly used. Flow cytometry is the best method to estimate DNA cvalue and ploidy levels in apples. In this study, DNA c-Value and ploidy level of Iranian apple varieties has been estimated by flow cytometry and propidum iodide staining…

 
Keyword(s): GENETIC DIVERSITY, GENOME SIZE, GERMPLASM, LOCAL CULTIVARS
 
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