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Paper Information

Journal:   JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY)   WINTER 2017 , Volume 30 , Number 4 ; Page(s) 634 To 642.
 
Paper: 

DETERMINING CYTOLOGICAL DEVELOPMENTS OF MICROSPORE IN FOUR VARIETIES OF TOMATO (LYCOPERSICUM ESCULENTUM MILL)

 
 
Author(s):  NAJIB R.*, FARSI M., MIRSHAMSI KAKHKI A., VESSAL S.R.
 
* DEPARTMENT OF BIOTECHNOLOGY AND PLANT BREEDING, FACULTY OF AGRICULTURE, FERDOWSI UNIVERSITY OF MASHHAD
 
Abstract: 

Introduction: Homozygous doubled haploid lines production through induction of androgenesis is a promising method to accelerate the classical breeding program. However, this technology is relatively under -developed in tomato so that improvements in methodology are required. Tomato (Lycopersicon esculentum Mill) is one of the most important vegetables which in addition of it is importance as a food, is utilized as a model plant for cytological and cytogenetic studies. Tomato breeding programs are often based on the production and selection of hybrid plants. To produce hybrid plants and application of features that is needed to breed pure lines with high specific combining abilities, new technologies such as doubled haploid production through induction of androgenesis can be an effective strategy to provide pure lines in tomato. One of the critical factors for induction of androgenesis in tomato is to use of microspores being in appropriate developmental stage. Cytological examination is one of the most accurate methods for determining the correct stage of microspore development. In this study, a number of characteristics were evaluated including the cytological properties of normal microspores development and pollen grains as well as the relationship between length of flower bud and anther length…

 
Keyword(s): ANDROGENESIS INDUCTION, HAPLOID, MICROSPORE DEVELOPMENT
 
References: 
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