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Paper Information

Journal:   TOLOO-E-BEHDASHT   APRIL-MAY 2016 , Volume 15 , Number 1 (55) ; Page(s) 1 To 9.
 
Paper: 

ISOLATING E.COLI BACTERIOPHAGE FROM RAW SEWAGE AND DETERMINING ITS SELECTIVITY TO THE HOST CELL

 
 
Author(s):  IMENI S.M., AKHAVAN SEPAHI A., SOLTAN DALLAL M.M.*
 
* FOOD MICROBIOLOGY RESEARCH CENTER, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

ntroduction: Bacteriophages are viruses that infect and destroy prokaryote cells, specifically the bacteria. They act too selective, so as each bacteriophage affects only on specific type of bacteria. Due to their specific features, bacteriophages can be used as an appropriate substitute for antibiotics in infectious diseases treatment. Therefore, this study aimed to isolate E. coli-specific bacteriophage from raw sewage.
Methods: Eight samples of raw sewage, each containing approximately 50 ml of raw sewage with 10 minute gap, were prepared from Zargandeh wastewater treatment plant, Tehran, Iran. The sewages were mixed with Brain-heart infusion medium (BHI) as a liquid culture medium in order to let the microorganisms grow. Incubation, purification and determination of bacteria were followed repeatedly to isolate the bacteriophage. Then it was tested on E.coli (ATCC 25922), Enterococcus faecalis (ATCC 19433), Staphylococcus aureus (ATCC 2392), and Yersinia enterocolitica (ATCC 9610) in order to determine the bacteriophage selectivity.
Results: The E.coli bacteriophages were successfully isolated from all the eight samples, that were completely able to lyse and destroy E.coli bacterial cells, though no effect was observed on other types of bacteria.
Conclusion: The study findings revealed that bacteriophages act selectively. Considering the raise of antibiotic resistance in the world, bacteriophages can serve as a good substitute for antibiotics in treating infectious diseases.

 
Keyword(s): ANTIBIOTICS, BACTERIOPHAGE, E.COLI, SEWAGE
 
References: 
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