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Paper Information

Journal:   IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS)   MARCH 2018 , Volume 43 , Number 2; Page(s) 208 To 213.
 
Paper: 

OPTIMIZATION OF THE STATIC HUMAN OSTEOBLAST/OSTEOCLAST CO-CULTURE SYSTEM

 
 
Author(s):  JOLLY JAMES JAM, CHIN KOK YONG, NUR FARHANA MOHD FOZI, ALIAS EKRAM, CHUA KIEN HUI, WAN HASAN WAN NURAINI, IMA NIRWANA SOELAIMAN*
 
* DEPARTMENT OF PHARMACOLOGY, FACULTY OF MEDICINE, UNIVERSITY KEBANGSAAN MALAYSIA, JALAN RAJA MUDA ABDUL AZIZ, 50300 KUALA LUMPUR, MALAYSIA
 
Abstract: 

Osteoblasts (OBs) and osteoclasts (OCs) are 2 major groups of bone cells. Their cell-to-cell interactions are important to ensure the continuity of the bone-remodeling process. Therefore, the present study was carried out to optimize an OB/OC co-culture system utilizing the human OB cell line hFOB 1.19 and OCs extracted from peripheral blood mononuclear cells (PBMNCs).
It was a 2-step procedure, involving the optimization of the OB culture and the co-culture of the OBs with PBMNCs at an optimum ratio. Firstly, pre-OBs were cultured to 90% confluency and the time required for differentiation was determined. OB differentiation was determined using the van Gieson staining to detect the presence of collagen and Alizarin Red for calcium.
Secondly, OBs and OCs were co-cultured at the ratios of 1 OC: 1 OB, 1 OC: 4 OBs, 2 OCs: 1 OB, and 1 OC: 2 OBs. Tartrateresistant acid phosphatase (TRAP) staining was used to detect the differentiation of the OCs. The results showed that collagen was present on day 1, whereas calcium was detected as early as day 3. Based on the result of TRAP staining, 1 OC: 2 OBs was taken as the most appropriate ratio. No macrophage colonystimulating factor and receptor activator of the nuclear factor-?B ligand were added because they were provided by the OBs. In conclusion, these optimization processes are vital as they ensure the exact time point and ratio of the OB/OC co-culture in order to produce a reliable and reproducible co-culture system.

 
Keyword(s): BONE AND BONES, COCULTURE TECHNIQUES, OSTEOBLASTS, OSTEOCLASTS, BONE REMODELING
 
 
References: 
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Click to Cite.
APA: Copy

JOLLY, J., & CHIN, K., & NUR FARHANA, M., & ALIAS, E., & CHUA, K., & WAN HASAN, W., & IMA NIRWANA, S. (2018). OPTIMIZATION OF THE STATIC HUMAN OSTEOBLAST/OSTEOCLAST CO-CULTURE SYSTEM. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), 43(2), 208-213. https://www.sid.ir/en/journal/ViewPaper.aspx?id=570711



Vancouver: Copy

JOLLY JAMES JAM, CHIN KOK YONG, NUR FARHANA MOHD FOZI, ALIAS EKRAM, CHUA KIEN HUI, WAN HASAN WAN NURAINI, IMA NIRWANA SOELAIMAN. OPTIMIZATION OF THE STATIC HUMAN OSTEOBLAST/OSTEOCLAST CO-CULTURE SYSTEM. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS). 2018 [cited 2021May14];43(2):208-213. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=570711



IEEE: Copy

JOLLY, J., CHIN, K., NUR FARHANA, M., ALIAS, E., CHUA, K., WAN HASAN, W., IMA NIRWANA, S., 2018. OPTIMIZATION OF THE STATIC HUMAN OSTEOBLAST/OSTEOCLAST CO-CULTURE SYSTEM. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), [online] 43(2), pp.208-213. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=570711.



 
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