Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES JOURNAL   2016 , Volume 18 , Number 3 ; Page(s) 48 To 56.
 
Paper: 

CONCURRENT ISOLATION OF VIRULENCE GENESETA, OPRL, GYRB IN PSEUDOMONAS AERUGINOSA CLINICAL SAMPLES FROM HOSPITALS IN KERMAN BY MULTIPLEX-PCR

 
 
Author(s):  KAMALI A., AMINI K.*
 
* MICROBIOLOGY DEPT., SAVEH BRANCH, ISLAMIC AZAD UNIVERSITY, SAVEH, I.R. IRAN
 
Abstract: 

Background and aims: Pseudomonas aeruginosa is an opportunistic pathogen causing nosocomial infections and mortality, especially in patients with a weakened immune system and leads to problems inherent resistance to antimicrobial agents in the treatment of infections. The aim of this study was to evaluate the prevalence of Pseudomonas aeruginosa virulence genes in Multiplex-PCR method.
Methods: A total of 60 samples of Pseudomonas aeruginosa including 40 samples from Passargad microbiology lab collection and 20 samples of hospitals in Kerman collected and cultured in specific media and were confirmed with biochemical tests. PCR reaction was done on all samples to identify virulence genes ETA, oprL, gyrB and the species 16SrDNA.
Results: Of the 60 isolates of Pseudomonas aeruginosa, 38 samples (63.3%) contains gene gyrB and oprL, 37 samples (61.6%) had the gene ETA, and all had 60 isolate (100%) gene 16SrDNA.
Conclusion: Considering the importance of rapid detection of this bacterium due to the incidence of resistance and presence of problems in biochemical methods, concurrent identification of multiple genes by Multiplex-PCR is a sensitive and accurative way in detecting the bacteria.

 
Keyword(s): MULTIPLEX PCR, VIRULENCE GENES, PSEUDOMONAS AERUOGINOSA
 
 
References: 
  • ندارد
  •  
 
Click to Cite.
APA: Copy

KAMALI, A., & AMINI, K. (2016). CONCURRENT ISOLATION OF VIRULENCE GENESETA, OPRL, GYRB IN PSEUDOMONAS AERUGINOSA CLINICAL SAMPLES FROM HOSPITALS IN KERMAN BY MULTIPLEX-PCR. SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES JOURNAL, 18(3 ), 48-56. https://www.sid.ir/en/journal/ViewPaper.aspx?id=570623



Vancouver: Copy

KAMALI A., AMINI K.. CONCURRENT ISOLATION OF VIRULENCE GENESETA, OPRL, GYRB IN PSEUDOMONAS AERUGINOSA CLINICAL SAMPLES FROM HOSPITALS IN KERMAN BY MULTIPLEX-PCR. SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES JOURNAL. 2016 [cited 2021May11];18(3 ):48-56. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=570623



IEEE: Copy

KAMALI, A., AMINI, K., 2016. CONCURRENT ISOLATION OF VIRULENCE GENESETA, OPRL, GYRB IN PSEUDOMONAS AERUGINOSA CLINICAL SAMPLES FROM HOSPITALS IN KERMAN BY MULTIPLEX-PCR. SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES JOURNAL, [online] 18(3 ), pp.48-56. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=570623.



 
 
Yearly Visit 33 Persian Abstract
 
Latest on Blog
Enter SID Blog