Paper Information

Journal:   NOVA BIOLOGICA REPERTA   2017 , Volume 4 , Number 3 #L0054; Page(s) 255 To 263.
 
Paper: 

EXTRACTION OF α-,β- AND γ-CHITOSAN FROM PORTUNUS SEGNIS, SEPIA PHARAONISAND ASPERGILLUS NIGER, AND COMPARISON OF THEIR ANTIMICROBIAL ACTIVITIES

 
 
Author(s):  KHAKSHOOR MOHAMMAD SADEGH, PAZOOKI JAMILEH*
 
* DEPARTMENT OF AQUATIC BIOLOGY AND BIOTECHNOLOGY, FACULTY OF LIFE SCIENCES AND BIOTECHNOLOGY, SHAHID BEHESHTI UNIVERSITY, G. C, TEHRAN, IRAN
 
Abstract: 

Three types of chitin as ? (exoskeleton of crustaceans), ? (cuttlebone of cephalopods) and ? (some fungal cell wall) exist in nature. Chitosan is the most important derivative of chitin, which has various biological activities. In this study, ?, ? and ?-chitosans were extracted fromPortunus segnis exoskeleton, Sepia pharonis cuttlebone and Aspergillus nigercell wall, respectively. Samples of crab and sepia from Bandar Abbas coastal waters were gathered in the summer of 2014 and samples ofAspergillus niger (PTCC 5223) were obtained from the Iranian Research Organization for Science and Technology (IROST). The amount of chitosan extracted from the Crab was significantly higher than other samples. Antimicrobial properties of these three types of chitosan were explored against 9 bacterial and 2 fungal strains using disc diffusion method. On average ? chitosan and ? chitosan revealed to have highest and lowest antimicrobial activities, respectively. Antibacterial properties of chitosan extracts were more than their antifungal properties. Gram negative bacteria as well as gram positive bacteria were sensitive to three types of chitosan. According to observed results, all three types of chitosans have good antimicrobial activities, and further investigations are suggested.

 
Keyword(s): BIOLOGICAL COMPOUNDS, CRUSTACEANS, MOLLUSKS, MICROORGANISMS
 
References: 
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