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Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   SUMMER 2018 , Volume 20 , Number 2; Page(s) 259 To 266.
 
Paper: 

PROSTAGLANDIN F-2α STIMULATES THE SECRETION OF VASCULAR ENDOTHELIAL GROWTH FACTOR AND INDUCES CELL PROLIFERATION AND MIGRATION OF ADIPOSE TISSUE DERIVED MESENCHYMAL STEM CELLS

 
 
Author(s):  DEEZAGI ABDOLKHALEG*, SHOMALI SAMIRA
 
* DEPARTMENT OF MOLECULAR MEDICINE AND BIOCHEMISTRY, NATIONAL INSTITUTE OF GENETIC ENGINEERING AND BIOTECHNOLOGY, KM. 17, KARAJ, TEHRAN
 
Abstract: 

Objective: Tissue engineering today uses factors that can induce differentiation of mesenchymal stem cells (MSCs) into other cell types. However, the problem of angiogenesis in this differentiated tissue remains an unresolved area of research interest. The aim of this study was to investigate the effects of prostaglandin F-2a (PGF-2 a) on the expression of vascular endothelial growth factor (VEGF) in human adipose tissue derived MSCs.
Materials and Methods: In this experimental research, human adipose tissue was digested using collagenase. The isolated MSCs cells were treated with PGF-2
a (up to 5 mg/ml) and incubated for 96 hours. Cell proliferation, secretion of VEGF and cell migration were spontaneously assayed by MTT, BrdU, ELISA, RT-PCR and scratching methods.
Results: Cell growth at 1.0, 2.5, 5
mg/ml of PGF-2 a was not significantly reduced compared to control cells, suggesting that these concentrations of PGF-2 a are not toxic to cell growth. The results of the BrdU incorporation assay indicated that, in comparison to untreated cells, BrdU incorporation was respectively 1.08, 1.96, 2.0 and 1.8 fold among cells treated with 0.1, 1.0, 2.5 and 5.0 mg/ml of PGF-2 a. The scratching test also demonstrated a positive influence on cell proliferation and migration. Cells treated with 1.0 mg/ml of PGF-2 a for 12 hours showed the highest relative migration and coverage in comparison to untreated cells. Quantitative VEGF ELISA and RTPCR results indicated an increase in VEGF expression and secretion in the presence of PGF-2 a. The amount of VEGF produced in response to 0.1, 1.0, 2.5 and 5.0 mg/ml of PGF-2 a was 62.4 ± 3.2 , 66.3 ± 3.7, 53.1 ± 2.6 and 49.0 ± 2.3 pg/ml, respectively, compared to the 35.2 ± 2.1 pg/ml produced by untreated cells.
Conclusion: Stimulation of VEGF secretion by PGF-2
a treated MSCs could be useful for the induction of angiogenesis in tissue engineering in vitro.

 
Keyword(s): ANGIOGENESIS, MESENCHYMAL STEM CELLS, PROSTAGLANDIN F2α, SCRATCHING, VASCULAR ENDOTHELIAL GROWTH FACTOR
 
 
References: 
 
Click to Cite.
APA: Copy

DEEZAGI, A., & SHOMALI, S. (2018). PROSTAGLANDIN F-2α STIMULATES THE SECRETION OF VASCULAR ENDOTHELIAL GROWTH FACTOR AND INDUCES CELL PROLIFERATION AND MIGRATION OF ADIPOSE TISSUE DERIVED MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH), 20(2), 259-266. https://www.sid.ir/en/journal/ViewPaper.aspx?id=570603



Vancouver: Copy

DEEZAGI ABDOLKHALEG, SHOMALI SAMIRA. PROSTAGLANDIN F-2α STIMULATES THE SECRETION OF VASCULAR ENDOTHELIAL GROWTH FACTOR AND INDUCES CELL PROLIFERATION AND MIGRATION OF ADIPOSE TISSUE DERIVED MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH). 2018 [cited 2021May08];20(2):259-266. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=570603



IEEE: Copy

DEEZAGI, A., SHOMALI, S., 2018. PROSTAGLANDIN F-2α STIMULATES THE SECRETION OF VASCULAR ENDOTHELIAL GROWTH FACTOR AND INDUCES CELL PROLIFERATION AND MIGRATION OF ADIPOSE TISSUE DERIVED MESENCHYMAL STEM CELLS. CELL JOURNAL (YAKHTEH), [online] 20(2), pp.259-266. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=570603.



 
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