Paper Information

Journal:   CELL JOURNAL (YAKHTEH)   SUMMER 2018 , Volume 20 , Number 2; Page(s) 231 To 243.
 
Paper: 

EFFECT OF (R)-(+) PULEGONE ON OVARIAN TISSUE; CORRELATION WITH EXPRESSION OF AROMATASE CYP19 AND OVARIAN SELECTED GENES IN MICE

 
 
Author(s):  SOULDOUZI ROHIYEH, RAZI MAZDAK, SHALIZAR JALALI ALI*, JALILZADEH AMIN GHADER, AMANI SAEEDEH
 
* DEPARTMENT OF BASIC SCIENCES, FACULTY OF VETERINARY MEDICINE, URMIA UNIVERSITY, URMIA, IRAN
 
Abstract: 

Objective: Pulegone (PGN) is a monoterpene ketone, whose metabolites exert several cytotoxic effects in various tissues. The present study was conducted in order to evaluate the (R)-(+) PGN-induced alterations in ovarian aromatization, proto-oncogenes and estrogen receptor? (ERa) and ER? receptors expressions.
Materials and Methods: In this experimental study, mature albino mice were divided into experimental (received 25 mg/kg, 50 mg/kg and 100 mg/kg PGN, orally for 35 days) and control (received 2% solution of Tween 80 as a PGN solvent, orally) groups. The mRNA levels of Er
a, Erb, p53, Bcl-2, and cytochrome p450 (Cyp19) as well as ovarian angiogenesis were analyzed through reverse transcription polymerase chain reaction and immunohistochemical techniques, respectively. Moreover, apoptosis of follicular cells, serum estrogen and progesterone levels and mRNA damage were investigated via using terminal transferase and biotin-16-dUTP staining, electrochemilunescence and fluorescent microscopy methods, respectively.
Results: The PGN reduced Er
a, Erb and Cyp19 expression at 50 mg/kg and 100 mg/kg doses, while significantly elevating p53 and reducing Bcl-2 expression. Finally, PGN impaired ovarian angiogenesis, increased apoptosis, elevated follicular atresia and reduced serum levels of estrogen and progesterone.
Conclusion: Chronic exposure to PGN (50 mg/kg and 100 mg/kg), severely affects ovarian aromatization, proto-oncogenes mRNA levels and expression of ERs.

 
Keyword(s): APOPTOSIS, AROMATIZATION, MICE, OVARY, PULEGONE
 
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