Paper Information

Journal:   IRANIAN SOUTH MEDICAL JOURNAL (ISMJ)   AUGUST-SEPTEMBER 2016 , Volume 19 , Number 3 #P0071; Page(s) 342 To 350.
 
Paper: 

EVALUATION OF IL-4 AND IFN-γ LEVELS IN CELL CULTURE SUPERNATANT OF ALLERGIC ASTHMA PATIENTS

 
 
Author(s):  ABEDIAN KENARI S., RAFAT MANESH A.*, AFTABI Y., NAJAFI M.
 
* DEPARTMENT OF IMMUNOLOGY, IMMUNOGENETICS RESEARCH CENTER, FACULTY OF IMMUNOLOGY, MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, SARI, IRAN
 
Abstract: 

Background: Allergic asthma is a chronic pulmonary disease with increasing of response and airways Inflammation. Measurement of cytokine changes caused by this disease can be effective to find control track or treatment of allergic asthma. In this study, we have investigated changes in IL-4 and IFN-g in patients with allergic asthma.
Materials & Methods: In this case-control study, 26 patients with allergic asthma and 26 healthy subject as control group were studied and they have been matched in terms of age and sex. After bloodletting, peripheral blood mononuclear cells were isolated and they were cultured and incubated at 37oC containing 5% CO2 for 72 hour. Supernatant collected and IFN-
g and IL-4 levels were measured by utilizing ELISA method.
Results: this study showed that IFN-
g level significantly have decreased in cell culture supernatant of patients in comparison with control group. (p<0.05). However IL-4 level and IL-4/IFN-g ratio increased significantly in cell culture supernatant of patients in comparison with control group (p<0.05).
Conclusion: According to results of this study, it seems that changing in IFN-
g and IL-4 levels have an important role in immune response against allergic asthma. Assessing IFN-g and IL-4 levels can be useful in study of immune response and clinical demonstration of allergic asthma, and it probably will be raised their balance control as a new perspective in the treatment of this disease.

 
Keyword(s): ALLERGIC ASTHMA, CYTOKINES, IFN-γ, IL-4
 
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