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Paper Information

Journal:   JOURNAL OF MICROBIAL WORLD   FALL 2017 , Volume 10 , Number 3 (32) ; Page(s) 231 To 242.
 
Paper: 

MOLECULAR IDENTIFICATION AND OPTIMIZATION OF KERATINASE PRODUCTION IN KERATIN-DEGRADING BACTERIA ISOLATED FROM SOIL POULTRY OF MARVDASHT CITY

 
 
Author(s):  KHODAYARI SOMAYEH, KAFILZADEH FARSHID*
 
* DEPARTMENT OF MICROBIOLOGY, JAHROM BRANCH, ISLAMIC AZAD UNIVERSITY, JAHROM, IRAN
 
Abstract: 

Background & Objectives: Feather is one of the environmental pollutants that about 90% of its weight is made of highly resistant creatine plates. A number of bacteria are able to produce a keratinase enzyme in the presence of keratin substrate. The purpose of this study was isolation and molecular identification of keratin- degrading bacteria from the soil of poultry farms around Marvdasht city in order to measure keratinase activity in superior creatine-degrading strains.
Material & Methods: In this study, 15 soil samples from poultry farms around Marvdasht city was collected. Seven bacterial isolates were cultured on a feather meal medium. Five isolates that showed clear degradation zone, were chosen for further chemical and molecular identification. The bacteria were sequenced, and a specific accession number in the gene bank was allocated to each of them as a new strain Then, all five isolates were assessed for the level of keratinase enzyme production.
Result: All isolates belonged to Bacillus species. All five isolates were able to completely degrade keratine. The most keratinase activity was reported 17.12 (ml/min) for Bacillus cereus SKH1.
Conclusion: Different species of Bacillus in this study showed the ability to produce a keratinase enzyme in the presence of keratin substrate. Investigating the level of keratinase activity in bacterial isolates showed that all have the potential treat feather waste.

 
Keyword(s): KERATINASE ENZYME, FEATHER-DEGRADING BACTERIA, BACILLUS CEREUS
 
 
References: 
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Click to Cite.
APA: Copy

KHODAYARI, S., & KAFILZADEH, F. (2017). MOLECULAR IDENTIFICATION AND OPTIMIZATION OF KERATINASE PRODUCTION IN KERATIN-DEGRADING BACTERIA ISOLATED FROM SOIL POULTRY OF MARVDASHT CITY. JOURNAL OF MICROBIAL WORLD, 10(3 (32) ), 231-242. https://www.sid.ir/en/journal/ViewPaper.aspx?id=569674



Vancouver: Copy

KHODAYARI SOMAYEH, KAFILZADEH FARSHID. MOLECULAR IDENTIFICATION AND OPTIMIZATION OF KERATINASE PRODUCTION IN KERATIN-DEGRADING BACTERIA ISOLATED FROM SOIL POULTRY OF MARVDASHT CITY. JOURNAL OF MICROBIAL WORLD. 2017 [cited 2021May08];10(3 (32) ):231-242. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569674



IEEE: Copy

KHODAYARI, S., KAFILZADEH, F., 2017. MOLECULAR IDENTIFICATION AND OPTIMIZATION OF KERATINASE PRODUCTION IN KERATIN-DEGRADING BACTERIA ISOLATED FROM SOIL POULTRY OF MARVDASHT CITY. JOURNAL OF MICROBIAL WORLD, [online] 10(3 (32) ), pp.231-242. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569674.



 
 
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