Paper Information

Journal:   THE JOURNAL OF TEHRAN UNIVERSITY HEART CENTER   2016 , Volume 11 , Number 3; Page(s) 123 To 138.
 
Paper: 

IMPROVEMENT OF HEART FAILURE BY HUMAN AMNIOTIC MESENCHYMAL STROMAL CELL TRANSPLANTATION IN RATS

 
 
Author(s):  RAZAVI TOUSI SEYED MOHAMMAD TAGHI, FAGHIHI MAHDIEH, NOBAKHT MALIHEH, MOLAZEM MOHAMMAD, KALANTARI ELHAM, DARBANDI AZAR AMIR, ABOUTALEB NAHID*
 
* PHYSIOLOGY RESEARCH CENTER, DEPARTMENT OF PHYSIOLOGY, IRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN. 6183-14155
 
Abstract: 

Background: Recently, stem cells have been considered for the treatment of heart diseases, but no marked improvement has been recorded. This is the first study to examine the functional and histological effects of the transplantation of human amniotic mesenchymal stromal cells (hAMSCs) in rats with heart failure (HF).
Methods: This study was conducted in the years 2014 and 2015.35 male Wistar rats were randomly assigned into 5 equal experimental groups (7 rats each) as 1- Control 2- Heart Failure (HF) 3- Sham 4- Culture media 5- Stem Cell Transplantation (SCT). Heart failure was induced using 170 mg/kg/d of isoproterenol subcutaneously injection in 4 consecutive days. The failure confirmed by the rat cardiac echocardiography on day 28. In SCT group, 3×106 cells in 150
ml of culture media were transplanted to the myocardium. At the end, echocardiographic and hemodynamic parameters together with histological evaluation were done.
Results: Echocardiography results showed that cardiac ejection fraction in HF group increased from 58.73±9% to 81.25±6.05% in SCT group (p value<0.001). Fraction shortening in HF group was increased from 27.53±8.58% into 45.5±6.91% in SCT group (p value<0.001). Furthermore, hAMSCs therapy significantly improved mean diastolic blood pressure, mean arterial pressure, left ventricular systolic pressure, rate pressure product, and left ventricular end-diastolic pressure compared to those in the HF group, with the values reaching the normal levels in the control group. A marked reduction in fibrosis tissue was also found in the SCT group (p value<0.001) compared with the animals in the HF group.
Conclusion: The transplantation of hAMSCs in rats with heart failure not only decreased the level of fibrosis but also conferred significant improvement in heart performance in terms of echocardiographic and hemodynamic parameters.

 
Keyword(s): HEART FAILURE, MESENCHYMAL STROMAL CELLS, CELL TRANSPLANTATION
 
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