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Paper Information

Journal:   JOURNAL OF MICROBIAL WORLD   SUMMER 2017 , Volume 10 , Number 2 (31) ; Page(s) 104 To 113.
 
Paper: 

MOLECULAR TYPING OF THE ACINETOBACTER BAUMANNII STRAINS ISOLATED FROM BLOOD INFECTIONS USING MULTI LOCUS SEQUENCE TYPING (MLST)

 
 
Author(s):  MOHAMMADI ZOHREH, MOMTAZ HASSAN*
 
* DEPARTMENT OF MICROBIOLOGY, SHAHREKORD BRANCH, ISLAMIC AZAD UNIVERSITY, SHAHREKORD, IRAN
 
Abstract: 

Background & Objectives: Acinetobacter baumannii is a Gram-negative coccobacillus that is widely distributed in nature and considered as one of the important causes of hospital infections. The present study was conducted to genotype Acinetobacter baumannii strains isolated from blood infections using Multi Locus Sequence Typing (MLST) method.
Materials & Methods: A total of 36 Acinetobacter baumannii strains were isolated from blood infection samples collected from Baqiatalah and Payambaran hospitals, Tehran, Iran. The PCR products obtained from amplification of seven housekeeping genes were sequenced. The nucleotide sequences of each gene in each isolate were queried against the reference sequence in the MLST database. In addition to characterization of the alleles specific to each gene, the sequence types (ST) of all isolates were determined.
Results: A total of 5 clones including ST25, ST136, ST307, ST327, and ST328 were identified in 36 isolates. ST of 2 isolates were not identified in MLST database. The identified STs were placed into 5 genetic clusters including A, B, C, D, and E.
Conclusion: Identifying an acceptable level of genetic diversity among the isolates using MLST technique shows that this method is useful for studying and typing of Acinetobacter baumannii isolates. Therefore, it is possible to cluster isolates with diverse origins in different groups.

 
Keyword(s): ACINETOBACTER BAUMANNII, HOUSEKEEPING GENES, MULTI LOCUS SEQUENCE TYPING, GENETIC CLONES, BLOOD INFECTION
 
 
References: 
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Click to Cite.
APA: Copy

MOHAMMADI, Z., & MOMTAZ, H. (2017). MOLECULAR TYPING OF THE ACINETOBACTER BAUMANNII STRAINS ISOLATED FROM BLOOD INFECTIONS USING MULTI LOCUS SEQUENCE TYPING (MLST). JOURNAL OF MICROBIAL WORLD, 10(2 (31) ), 104-113. https://www.sid.ir/en/journal/ViewPaper.aspx?id=569607



Vancouver: Copy

MOHAMMADI ZOHREH, MOMTAZ HASSAN. MOLECULAR TYPING OF THE ACINETOBACTER BAUMANNII STRAINS ISOLATED FROM BLOOD INFECTIONS USING MULTI LOCUS SEQUENCE TYPING (MLST). JOURNAL OF MICROBIAL WORLD. 2017 [cited 2021May12];10(2 (31) ):104-113. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569607



IEEE: Copy

MOHAMMADI, Z., MOMTAZ, H., 2017. MOLECULAR TYPING OF THE ACINETOBACTER BAUMANNII STRAINS ISOLATED FROM BLOOD INFECTIONS USING MULTI LOCUS SEQUENCE TYPING (MLST). JOURNAL OF MICROBIAL WORLD, [online] 10(2 (31) ), pp.104-113. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569607.



 
 
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