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Journal:   BIOLOGICAL JOURNAL OF MICROORGANISM   SUMMER 2017 , Volume 6 , Number 22 ; Page(s) 127 To 142.
 
Paper: 

SYNTHESIS OF POLY (3-HYDROXYBUTYRATE) USING ENZYMES AZOSPIRILLUM BRASILENSE IN VITRO

 
 
Author(s):  ABBASI SOHEILA, EMTIAZI GITI*, ROGHANIAN RASOUL
 
* UNIVERSITY OF ISFAHAN, IRAN
 
Abstract: 

Introduction: Polyhydroxyalkaniates (PHAS) are bacterial polymers that are formed as naturally occurring storage polyesters by a wide range of microorganisms usually under unbalanced growth conditions. Mechanical properties of PHAS make them suitable replacements for petrochemically produced bulk plastics (polyethylene, polypropylene etc.), but in contrast to these commodity plastics PHA are completely degradable to carbon dioxide and water through natural microbiological mineralization. PHAS can be produced by biotechnological processes under controlled conditions. Polyhydroxybotyric acid (PHB) was the first of the PHAS discovered and is the most abundant polyester found in bacteria.
Materials and methods: First, a rich bacterial suspension prepared from poly(3-hydroxybutyrate), then the cells were broken down and ensure the absence of live cells. The cell free enzymes were added to M9 medium. Then the sample turbidity was measured at a wavelength of 600 nm and incubated at 30°C and centrifiuged at 120rpm. Next, the amount of PHB as well as solution turbidity were measured and compared with control. The physical and chemical analyzes of extracted PHB samples such as UV absorbtion, FTIR, HPLC and HNMR assesed and compared with standard sample.
Results: In twelve days this experiment was conducted, from the third day, the amount of polyhydroxybutyrate was measurable and the highest polyhydroxybutyrate was gained on the tenth day. The level of PHB was constant. Moreover, the level of glucose was decreased gradually till the tenth day. By Physico-chemical analysis, production of polyhydroxybutyrate was approved.
Discussion and conclusion: In this experiment, the reaction efficiency was 37.5 percent. Probably the rest of glucose could be changed to other intermediate compounds by other enzymes in the sample. Therefore, by purification of the enzyme, usage of specific substrate and optimization of conditions outside the cell could help the production of polyhydroxybutyrate continually. Production is very cost-effective, by this method.

 
Keyword(s): PHB, POLYESTER, AZOSPIRILCOMPARATIVE PRODUCTION OF CELLULASES BY MUTANTS OF TRICHODERMA PARCERAMOSUME PTCC5140
 
 
References: 
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Citations: 
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APA: Copy

ABBASI, S., & EMTIAZI, G., & ROGHANIAN, R. (2017). SYNTHESIS OF POLY (3-HYDROXYBUTYRATE) USING ENZYMES AZOSPIRILLUM BRASILENSE IN VITRO. BIOLOGICAL JOURNAL OF MICROORGANISM, 6(22 ), 127-142. https://www.sid.ir/en/journal/ViewPaper.aspx?id=569604



Vancouver: Copy

ABBASI SOHEILA, EMTIAZI GITI, ROGHANIAN RASOUL. SYNTHESIS OF POLY (3-HYDROXYBUTYRATE) USING ENZYMES AZOSPIRILLUM BRASILENSE IN VITRO. BIOLOGICAL JOURNAL OF MICROORGANISM. 2017 [cited 2021July27];6(22 ):127-142. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569604



IEEE: Copy

ABBASI, S., EMTIAZI, G., ROGHANIAN, R., 2017. SYNTHESIS OF POLY (3-HYDROXYBUTYRATE) USING ENZYMES AZOSPIRILLUM BRASILENSE IN VITRO. BIOLOGICAL JOURNAL OF MICROORGANISM, [online] 6(22 ), pp.127-142. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569604.



 
 
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