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Paper Information

Journal:   BIOLOGICAL JOURNAL OF MICROORGANISM   SUMMER 2017 , Volume 6 , Number 22 ; Page(s) 89 To 99.
 
Paper: 

RAPID MOLECULAR DETECTION OF CITRUS BROWN SPOT DISEASE USING ACT GENE IN ALTERNARIA ALTERNATA

 
 
Author(s):  MOGHIMI HAMID*, MORADI AMIR, HAMEDI JAVAD
 
* SCHOOL OF BIOLOGY, COLLEGE OF SCIENCE, UNIVERSITY OF TEHRAN, TEHRAN, IRAN
 
Abstract: 

Introduction: Using rapid detection methods is important for detection of plant pathogens and also prevention through spreading pests in agriculture. Citrus brown spot disease caused by pathogenic isolates of Alternaria alternata is a common disease in Iran.
Materials and methods: In this study, for the first time a PCR based molecular method was used for rapid diagnosis of brown spot disease. Nine isolates of A. Alternata were isolated in PDA medium from different citrus gardens. The plant pathogenic activity was examined in tangerine leaves for isolates. Results showed that these isolates are the agents of brown spot disease. PCR amplification of specific ACT-toxin gene was performed for DNA extracted from A. alternata isolates, with 11 different fungal isolates as negative controls and 5 DNA samples extracted from soil.
Results: Results showed that A. alternata, the causal agent of brown spot disease, can be carefully distinguished from other pathogenic agents by performing PCR amplification with specific primers for ACT toxin gene. Also, the results from Nested-PCR method confirmed the primary reaction and the specificity of A. alternata for brown spot disease. PCR results to control samples of the other standard fungal isolates, showed no amplification band. In addition, PCR with the DNA extracted from contaminated soils confirmed the presence of ACT toxin gene.
Discussion and conclusion: Molecular procedure presented here can be used in rapid identification and prevention of brown spot infection in citrus gardens all over the country.

 
Keyword(s): BROWN SPOT, MOLECULAR IDENTIFICATION, ALTERNARIA ALTERNATE, ACT TOXIN
 
 
References: 
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Citations: 
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APA: Copy

MOGHIMI, H., & MORADI, A., & HAMEDI, J. (2017). RAPID MOLECULAR DETECTION OF CITRUS BROWN SPOT DISEASE USING ACT GENE IN ALTERNARIA ALTERNATA. BIOLOGICAL JOURNAL OF MICROORGANISM, 6(22 ), 89-99. https://www.sid.ir/en/journal/ViewPaper.aspx?id=569585



Vancouver: Copy

MOGHIMI HAMID, MORADI AMIR, HAMEDI JAVAD. RAPID MOLECULAR DETECTION OF CITRUS BROWN SPOT DISEASE USING ACT GENE IN ALTERNARIA ALTERNATA. BIOLOGICAL JOURNAL OF MICROORGANISM. 2017 [cited 2021July31];6(22 ):89-99. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569585



IEEE: Copy

MOGHIMI, H., MORADI, A., HAMEDI, J., 2017. RAPID MOLECULAR DETECTION OF CITRUS BROWN SPOT DISEASE USING ACT GENE IN ALTERNARIA ALTERNATA. BIOLOGICAL JOURNAL OF MICROORGANISM, [online] 6(22 ), pp.89-99. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569585.



 
 
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