Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   BIOLOGICAL JOURNAL OF MICROORGANISM   SUMMER 2017 , Volume 6 , Number 22 ; Page(s) 59 To 76.
 
Paper: 

AN IN-SILICO INVESTIGATION ON THE STRUCTURE, FUNCTION AND HOMOLOGOUS SEQUENCES OF THE ENZYMES AND PROTEINS INVOLVED IN THE PRODUCTION AND ACCUMULATION OF THE LIPIDS IN BIODIESEL RESOURCES

 
 
Author(s):  FARMANBAR NAJMEH, HADDAD MASHADRIZEH ALIAKBAR*, HEMMAT JAFAR
 
* CELL AND MOLECULAR RESEARCH GROUP, INSTITUTE OF BIOTECHNOLOGY, FERDOWSI UNIVERSITY OF MASHHAD, MASHHAD, IRAN
 
Abstract: 

Introduction: Biodiesel as a biofuel, with renewable, biodegradable and free of polycyclic aromatic hydrocarbons properties, could be derived from fatty acids of the cells of the animals, plants, algae and bacteria. So, a wide range of approaches have been considered to increase the oil production in biodiesel resources. In this regard, biotechnology approaches can provide new solutions based on application of molecular mechanisms.
Materials and methods: A comprehensive survey on molecular mechanisms and key enzymes which are involved in the production and accumulation of the lipids in biodiesel resources have been considered based on literature study and in-silico investigation. In-silico investigation has been performed via InterProScan 5، Motif scan، Conserved Domain، ProtParam، TMHMM، GC content calculator، NetNGlyc، NetPhos، Sulfinator، Protein Blastand MEGA6 programs for characterizing the structure, functions and homology survey of the selected sequences.
Results: Our survey led to the introduced diacyl glycerol acyl transferase (DGAT), waxester synthase/diacyl glycerol acyltransferase (WS/DGAT), oleosin, MLDP and TadA as the effective enzymes and proteins in lipid production and accumulation in selected biodiesel organisms. An investigation on the structure of the corresponding genes of the selected enzymes, led to reveal their various features in the length, GC content as well as in intronic properties. On the other hand, this characterizing on the selected enzymes and proteins disclosed post-translational modifications in all of them, as well as their localization in the cells. Moreover MBOAT, DAGAT, UPF0089, Oleosin and apolipo protein have been revealed in their context as critical domains. On the other hand, homology survey of the selected enzymes and proteins led to introduce the Verniciafordii, Ricinuscommunis, Dunaliella parva , Thalassiosira pseudonana,  Saitoella complicata, Rhodococcus imtechensis and Rhodococcus wratislaviensis species, as new sources of biodiesel with possible capability for lipid production.
Discussion and conclusion: Overall, this survey provides a series of motifs and domains in biodiesel process, as well as introducing several organisms with potency in biodiesel production, which could be more examined in an experimental condition.

 
Keyword(s): BIODIESEL, OLEAGINOUS ORGANISMS, DGAT, WS/DGAT, OLEOSIN, MLDP, TADA
 
 
References: 
  • ندارد
  •  
 
Click to Cite.
APA: Copy

FARMANBAR, N., & HADDAD MASHADRIZEH, A., & HEMMAT, J. (2017). AN IN-SILICO INVESTIGATION ON THE STRUCTURE, FUNCTION AND HOMOLOGOUS SEQUENCES OF THE ENZYMES AND PROTEINS INVOLVED IN THE PRODUCTION AND ACCUMULATION OF THE LIPIDS IN BIODIESEL RESOURCES. BIOLOGICAL JOURNAL OF MICROORGANISM, 6(22 ), 59-76. https://www.sid.ir/en/journal/ViewPaper.aspx?id=569573



Vancouver: Copy

FARMANBAR NAJMEH, HADDAD MASHADRIZEH ALIAKBAR, HEMMAT JAFAR. AN IN-SILICO INVESTIGATION ON THE STRUCTURE, FUNCTION AND HOMOLOGOUS SEQUENCES OF THE ENZYMES AND PROTEINS INVOLVED IN THE PRODUCTION AND ACCUMULATION OF THE LIPIDS IN BIODIESEL RESOURCES. BIOLOGICAL JOURNAL OF MICROORGANISM. 2017 [cited 2021May07];6(22 ):59-76. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569573



IEEE: Copy

FARMANBAR, N., HADDAD MASHADRIZEH, A., HEMMAT, J., 2017. AN IN-SILICO INVESTIGATION ON THE STRUCTURE, FUNCTION AND HOMOLOGOUS SEQUENCES OF THE ENZYMES AND PROTEINS INVOLVED IN THE PRODUCTION AND ACCUMULATION OF THE LIPIDS IN BIODIESEL RESOURCES. BIOLOGICAL JOURNAL OF MICROORGANISM, [online] 6(22 ), pp.59-76. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569573.



 
 
Yearly Visit 50 Persian Abstract
 
Latest on Blog
Enter SID Blog