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Paper Information

Journal:   SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL   FALL 2017 , Volume 13 , Number 3 ; Page(s) 75 To 85.
 
Paper: 

TYPING OF CLOSTRIDIUM PERFRINGENS ISOLATES FROM SHEEP IN AHVAZ

 
 
Author(s):  GHARIBI D.*, GHORBANPOUR M., HAJI HAJIKOLAEI M.R., MAHMOUDI KOHI Z.
 
* DEPARTMENT OF PATHOBIOLOGY, FACULTY OF VETERINARY MEDICINE, SHAHID CHAMRAN UNIVERSITY OF AHVAZ, AHVAZ, IRAN
 
Abstract: 

Clostridium perfringensis an important pathogen which causes numerous different diseases in animals. This bacterium is classified into five different types; A, B, C, D and E.
Clostridium perfringenstype D cause enterotoxemia in animals. The aim of this study was to identify different types ofClostridium perfringens in sheep in Ahvaz. For this purpose, 369 fecal samples were randomly collected from the sheep from a different area. After processing and culturing samples in blood agar containing neomycin, Clostridium perfringens suspected isolates were identified morphologically and biochemically. According to biochemical tests, from 369 feces samples, 56 isolates obtained and identified as Clostridium perfringens. For molecular confirmation of the isolate, the DNA of the isolates was extracted and the isolates were verified asClostridium perfringens by PCR using specific primers for alfa toxin. Theresults showed that 54 isolated from 56 isolated confirmed asClostridium perfringens.
Typing of these isolates was done by PCR using specific primers for 5 different exotoxins of this bacterium. The results showed that 10 isolates (18.51%) were type A and 4 (7.4%), 24 (44.44%) and 16 isolates (29.62%) were type B, type C and type D respectively. Thus the most prevalent types ofClostridium perfringens were type C and D. In the present study, all isolates were negative for iota toxin and enterotoxin gene.

 
Keyword(s): CLOSTRIDIUM PERFRINGENS, GENOTYPE, PCR, SHEEP, AHVAZ
 
 
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Click to Cite.
APA: Copy

GHARIBI, D., & GHORBANPOUR, M., & HAJI HAJIKOLAEI, M., & MAHMOUDI KOHI, Z. (2017). TYPING OF CLOSTRIDIUM PERFRINGENS ISOLATES FROM SHEEP IN AHVAZ. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 13(3 ), 75-85. https://www.sid.ir/en/journal/ViewPaper.aspx?id=569544



Vancouver: Copy

GHARIBI D., GHORBANPOUR M., HAJI HAJIKOLAEI M.R., MAHMOUDI KOHI Z.. TYPING OF CLOSTRIDIUM PERFRINGENS ISOLATES FROM SHEEP IN AHVAZ. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL. 2017 [cited 2021May14];13(3 ):75-85. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569544



IEEE: Copy

GHARIBI, D., GHORBANPOUR, M., HAJI HAJIKOLAEI, M., MAHMOUDI KOHI, Z., 2017. TYPING OF CLOSTRIDIUM PERFRINGENS ISOLATES FROM SHEEP IN AHVAZ. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, [online] 13(3 ), pp.75-85. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569544.



 
 
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