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Paper Information

Journal:   SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL   FALL 2017 , Volume 13 , Number 3 ; Page(s) 56 To 66.
 
Paper: 

DETERMINATION OF GENETIC DIVERSITY AND SUSCEPTIBILITY OF TRICHOPHYTON MENTAGROPHYTES ISOLATES AGAINST ANTIFUNGAL EFFECTS OF ETHANOLIC EXTRACT OF ALLIUM JESDIANUM

 
 
Author(s):  RAZZAGHI KHEZERLO S., SHARIFZADEH A.*, SOLTANI M., SHOKRI H., KHOSRAVI A.
 
* DEPARTMENT OF MICROBIOLOGY, FACULTY OF VETERINARY MEDICINE, UNIVERSITY OF TEHRAN, TEHRAN, IRAN
 
Abstract: 

Dermatophytes are a group of fungi with keratinophilic properties. They are able to invade epidermis, especially stratum corneum, by the production of proteolytic enzymes such as keratinase.
Trichophyton mentagrophytes (T. mentagrophytes) is a common cosmopolitan dermatophyte species. This species has different varieties based on genetic characteristics. In this study genetic diversity of 20 clinical isolates ofT. mentagrophytes and their susceptibility to ethanolic extract ofAllium jesdianum (A. jesdianum) were evaluated. Genetic diversity of the isolates was assessed by Random Amplified Polymorphic DNA (RAPD) method using 4 primers including OPN16, A08, OPD18 and R28. Then, susceptibility ofT. mentagrophytes isolates toA. jesdianum extract was determined by standard microdilution broth method. The MIC values of the isolates were between 6.1 and 49.3 mg/ml (mean MIC: 20.3 mg/ml), whereas the MFC values of the isolates were between 24.6 and 49.3 mg/ml (mean MFC: 40.6 mg/ml). Twenty eight propagated DNA fragments were achieved in RAPD-PCR reaction. The most propagated DNA fragments (9 bands) were related to the OPD18 primer. The R28 primer showed the least propagated DNA fragments (5 bands). Drawn dendrogram identified 3 main groups among 20T. mentagrophytes isolates according to all primers in 65% genetic distance. Most ofT. mentagrophytes isolates (75%) were placed in group 2 and 100% genetical similarity was only seen between 2 isolates. It was concluded that allT.
mentagrophytesgenotypes were susceptible to A. jesdianum. The correlation between antifungal susceptibility andT. mentagrophytes genotype may be of potential therapeutic significance and larger studies are needed to prove this finding.

 
Keyword(s): DERMATOPHYTE, TRICHOPHYTON MENTAGROPHYTES, ANTIFUNGAL SUSCEPTIBILITY, GENETIC VARIATION, ALLIUM JESDIANUM
 
References: 
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