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Paper Information

Journal:   BIOLOGICAL JOURNAL OF MICROORGANISM   WINTER 2017 , Volume 5 , Number 20 ; Page(s) 71 To 80.
 
Paper: 

CHARACTERIZATION AND EVALUATION OF CATECHOL OXYGENASES BY TWELVE BACTERIA, ISOLATED FROM OIL CONTAMINATED SOILS IN MALAYSIA

 
 
Author(s):  TAVAKOLI AREZOO*, HAMZAH AINON
 
* ISLAMIC AZAD UNIVERSITY EGHLID BRANCH, EGHLID, IRAN
 
Abstract: 

Introduction: Catechol is a common intermediate compound in aromatic degradation process. Some microorganisms have this potentiality to degrade aromatic hydrocarbons by catechol dioxygenases to less toxic compounds with ability of entering the tricarboxylic acid cycle. In the present study, the catechol oxygenase activity was measured for 12 crude oil degrader bacteria.
Materials and methods: Catechol oxygenase activity of two enzymes includes catechol 1, 2 dioxygenase and catechol 2, 3 dioxygenase were determined using spectrophotometer at 260 nm and 375 nm, respectively.
Results: The highest enzyme activity for catechol 1, 2 dioxygenase by Bacillus cereus UKMP-6G was (0.07 U/mL) and about catechol 2, 3 dioxygenase was 0.031 U/mL by Rhodococcus ruber UKMP-5M during the first minute of incubation. Catechol 1, 2 dioxygenase and catechol 2, 3 dioxygenase followed the ortho and meta pathway, respectively.
Discussion and conclusion: The enzyme assay results showed that among 12 examined bacteria, only R. ruber UKMP-5M has the ability to use meta pathway for degradation and produce 2-hydroxymuconic acid. The other isolates use ortho pathway and create cis, cis -muconic acid.

 
Keyword(s): CATECHOL OXYGENASE, DEGRADATION, TOLUENE, ENZYME ASSAY
 
 
References: 
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APA: Copy

TAVAKOLI, A., & HAMZAH, A. (2017). CHARACTERIZATION AND EVALUATION OF CATECHOL OXYGENASES BY TWELVE BACTERIA, ISOLATED FROM OIL CONTAMINATED SOILS IN MALAYSIA. BIOLOGICAL JOURNAL OF MICROORGANISM, 5(20 ), 71-80. https://www.sid.ir/en/journal/ViewPaper.aspx?id=569505



Vancouver: Copy

TAVAKOLI AREZOO, HAMZAH AINON. CHARACTERIZATION AND EVALUATION OF CATECHOL OXYGENASES BY TWELVE BACTERIA, ISOLATED FROM OIL CONTAMINATED SOILS IN MALAYSIA. BIOLOGICAL JOURNAL OF MICROORGANISM. 2017 [cited 2021May12];5(20 ):71-80. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569505



IEEE: Copy

TAVAKOLI, A., HAMZAH, A., 2017. CHARACTERIZATION AND EVALUATION OF CATECHOL OXYGENASES BY TWELVE BACTERIA, ISOLATED FROM OIL CONTAMINATED SOILS IN MALAYSIA. BIOLOGICAL JOURNAL OF MICROORGANISM, [online] 5(20 ), pp.71-80. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569505.



 
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