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Journal:   BIOLOGICAL JOURNAL OF MICROORGANISM   WINTER 2017 , Volume 5 , Number 20 ; Page(s) 9 To 16.
 
Paper: 

Antibiotic Resistance Of Verotoxigenic Escherichia Coli Isolated From Vegetables
 
 
Author(s):  Bonyadian Mojtaba*, Moshtaghi Hamdallah, Mohamad Taghipour Laya
 
* INSTITUTE OF ZOONOSES RESEARCH, SHAHREKORD UNIVERSITY, IRAN
 
Abstract: 
Introduction: Human gastrointestinal disease caused by verotoxigenic Escherichia coli has been diagnosed for recent decades. Escherichia coli O157: H7 is the most important serotype of verotoxigenic Escherichia coli that cause hemolytic uremic syndrome and hemorrhagic colitis in humans. This study was conducted to determine the occurrence of verotoxigenic E. coli and antibiotic resistance of the isolates from vegetables.Materials and methods: A total of 500 fresh vegetable samples were collected randomly from retail shops in Shahrekord, Iran. E. coli was isolated and identified using bacteriological and biochemical tests. PCR method was used to identify the rbfE, stx1, stx2 and eae genes. Also, antibiotic resistance of the isolates was determined by disk diffusion method.Results: The results represented that among 25 isolates possess virulence genes, 40, 12 and 4% of the isolates contained eaeA, STx2, and both genes, respectively. But none of them contained H7, STx1, and rfbE genes. The antibiotic resistance pattern demonstrated that the isolates were highly resistant to Gentamycin and cefotoxime.Discussion and conclusion: The results of this study showed that the presence of verotoxigenic E.coli in vegetables; and high resistance of the isolates to antibiotics could be hazardous for public health.
 
Keyword(s): VEROTOXIGENIC E.COLI, VIRULENCE GENES, ANTIBIOTIC RESISTANCE, VEGETABLES
 
 
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APA: Copy

BONYADIAN, M., & MOSHTAGHI, H., & MOHAMAD TAGHIPOUR, L. (2017). ANTIBIOTIC RESISTANCE OF VEROTOXIGENIC ESCHERICHIA COLI ISOLATED FROM VEGETABLES. BIOLOGICAL JOURNAL OF MICROORGANISM, 5(20 ), 9-16. https://www.sid.ir/en/journal/ViewPaper.aspx?id=569494



Vancouver: Copy

BONYADIAN MOJTABA, MOSHTAGHI HAMDALLAH, MOHAMAD TAGHIPOUR LAYA. ANTIBIOTIC RESISTANCE OF VEROTOXIGENIC ESCHERICHIA COLI ISOLATED FROM VEGETABLES. BIOLOGICAL JOURNAL OF MICROORGANISM. 2017 [cited 2022May26];5(20 ):9-16. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569494



IEEE: Copy

BONYADIAN, M., MOSHTAGHI, H., MOHAMAD TAGHIPOUR, L., 2017. ANTIBIOTIC RESISTANCE OF VEROTOXIGENIC ESCHERICHIA COLI ISOLATED FROM VEGETABLES. BIOLOGICAL JOURNAL OF MICROORGANISM, [online] 5(20 ), pp.9-16. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569494.
 
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