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Paper Information

Journal:   IRANIAN JOURNAL OF VIROLOGY   2015 , Volume 9 , Number 1; Page(s) 7 To 12.
 
Paper: 

REPLICATION KINETIC OF INFECTIOUS LARYNGOTRACHEITIS VIRUS IN EMBRYONIC CHICKEN NEURAL STEM CELL

 
 
Author(s):  JAMSHIDI NAVROUD Z., SHAHSAVANDI S.*, FIROUZI M.
 
* RAZI VACCINE AND SERUM RESEARCH INSTITUTE, KARAJ, IRAN
 
Abstract: 

Background and Aims: Infectious laryngotracheitis virus (ILTV) infection causes a highly contagious respiratory disease in poultry which leads to significant economic losses in the poultry industry. The genomic and antigenic characteristics between virulent and vaccine strains of ILTV are very similar. The ability of embryonated chicken neural stem (CNS) cell for differential diagnosis of ILTV strains was also evaluated.
Materials and Methods: CNS cell cultures were inoculated with an ILTV vaccine stain and a virulent strain at different multiplicity of infections (MOI). The replication and growth kinetics of the viruses were evaluated by estimation of suitable time of infection, production of virus infectivity titers, and amplification of ICP4 gene in collected samples.
Results: Based on PCR and virus titration assays, the ILTV strains were able to enter CNS cells and replicate efficiently at all MOIs used. The virus titers and tracing of ICP4 data were found to be similar, but marked cytopathic effect (CPE) was only detected in ILTV vaccine stain infection as compared with virulent strain.
Conclusion: The neural stem cell had a higher growth potential and infectivity for ILTV strains, which will be of advantage in differential diagnosis.

 
Keyword(s): INFECTIOUS LARYNGOTRACHEITIS VIRUS, EMBRYONIC CHICKEN NEURAL STEM CELL, REPLICATION KINETIC, CYTOPATHIC EFFECT
 
 
References: 
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APA: Copy

JAMSHIDI NAVROUD, Z., & SHAHSAVANDI, S., & FIROUZI, M. (2015). REPLICATION KINETIC OF INFECTIOUS LARYNGOTRACHEITIS VIRUS IN EMBRYONIC CHICKEN NEURAL STEM CELL. IRANIAN JOURNAL OF VIROLOGY, 9(1), 7-12. https://www.sid.ir/en/journal/ViewPaper.aspx?id=569478



Vancouver: Copy

JAMSHIDI NAVROUD Z., SHAHSAVANDI S., FIROUZI M.. REPLICATION KINETIC OF INFECTIOUS LARYNGOTRACHEITIS VIRUS IN EMBRYONIC CHICKEN NEURAL STEM CELL. IRANIAN JOURNAL OF VIROLOGY. 2015 [cited 2021May18];9(1):7-12. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569478



IEEE: Copy

JAMSHIDI NAVROUD, Z., SHAHSAVANDI, S., FIROUZI, M., 2015. REPLICATION KINETIC OF INFECTIOUS LARYNGOTRACHEITIS VIRUS IN EMBRYONIC CHICKEN NEURAL STEM CELL. IRANIAN JOURNAL OF VIROLOGY, [online] 9(1), pp.7-12. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569478.



 
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