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Paper Information

Journal:   IRANIAN JOURNAL OF VIROLOGY   2015 , Volume 9 , Number 1; Page(s) 1 To 6.
 
Paper: 

DEVELOPMENT OF QRT-PCR TEST FOR QUANTIFICATION OF RUBELLA VIRUS IN COMMERCIALLY AVAILABLE VACCINES

 
 
Author(s):  SHEIKHOLESLAMI F., KHANAHMAD H., AJORLOO M., AHANGARI COHAN R., KARIMI Z., NOROUZIAN D.*
 
* PHD; PILOT NANOBIOTECHNOLOGY DEPARTMENT, PASTEUR INSTITUTE OF IRAN, TEHRAN, IRAN
 
Abstract: 

Background and Aims: Determination of virions in live vaccines, especially multivalent vaccines is considered as a critical point and in-process of quality control (IPQC) test of vaccine production. Rapid tests, like real time PCR, are more appropriate when the production occurs at industrial scale because of the amounts of starting materials and the excess of consumed time required. In the current study, a real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was developed for Rubella virus (RV) and tested on a commercially available MMR vaccine.
Materials and Methods: The primers and Taq Man probe were designed by Gene runner version 5.0.63 software. Concentrations, as well as reaction temperatures were optimized to establish an efficient qRT-PCR assay for RV RNA. A RV-specific PCR amplicon (109 bp), conserved in all species, was made as an external standard to evaluate the linearity, amplification efficiency, analytical sensitivity and reproducibility of the qRT-PCR.
Results: The real time quantitative assay was shown to have good linearity (R2=0.998), high amplification efficiency (E=96.18%), and high sensitivity (7×102 copies/7
ml) for tested vaccine.
Conclusion: The established qRT-PCR method is a simple, rapid, quantitative, highly specific and sensitive assay for quantification of RV RNA copy numbers in IPQC tests at industrial scale.

 
Keyword(s): RUBELLA VIRUS, MUMPS MEASLES RUBELLA (MMR) VACCINE, QUANTITATIVE REAL-TIME REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION
 
 
References: 
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Click to Cite.
APA: Copy

SHEIKHOLESLAMI, F., & KHANAHMAD, H., & AJORLOO, M., & Ahangari Cohan, R., & KARIMI, Z., & NOROUZIAN, D. (2015). DEVELOPMENT OF QRT-PCR TEST FOR QUANTIFICATION OF RUBELLA VIRUS IN COMMERCIALLY AVAILABLE VACCINES. IRANIAN JOURNAL OF VIROLOGY, 9(1), 1-6. https://www.sid.ir/en/journal/ViewPaper.aspx?id=569465



Vancouver: Copy

SHEIKHOLESLAMI F., KHANAHMAD H., AJORLOO M., Ahangari Cohan R., KARIMI Z., NOROUZIAN D.. DEVELOPMENT OF QRT-PCR TEST FOR QUANTIFICATION OF RUBELLA VIRUS IN COMMERCIALLY AVAILABLE VACCINES. IRANIAN JOURNAL OF VIROLOGY. 2015 [cited 2021May11];9(1):1-6. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569465



IEEE: Copy

SHEIKHOLESLAMI, F., KHANAHMAD, H., AJORLOO, M., Ahangari Cohan, R., KARIMI, Z., NOROUZIAN, D., 2015. DEVELOPMENT OF QRT-PCR TEST FOR QUANTIFICATION OF RUBELLA VIRUS IN COMMERCIALLY AVAILABLE VACCINES. IRANIAN JOURNAL OF VIROLOGY, [online] 9(1), pp.1-6. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569465.



 
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