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Paper Information

Journal:   NOVELTY IN BIOMEDICINE   2016 , Volume 4 , Number 4; Page(s) 142 To 146.
 
Paper: 

CLONING AND EXPRESSION OF LUTEINIZING HORMONE SUBUNITS IN CHINESE HAMSTER OVARY CELL LINE

 
 
Author(s):  SOLEIMANIFAR ZEINAB, BANDEHPOUR MOJGAN*, KAZEMI BAHRAM
 
* DEPARTMENT OF BIOTECHNOLOGY, SCHOOL OF ADVANCED TECHNOLOGIES IN MEDICINE, SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Background: Luteinizing hormone (LH) was secreted by the stimulating cells of the testes and ovaries in the anterior pituitary gland. The application of this hormone is in the treatment of men and women with infertility and amenorrhea respectively.
Materials and Methods: In the present study the alpha and beta subunits of human LH gene were cloned into the pEGFP-N1 expression vector and produced the recombinant LH hormone in Chinese hamster ovary (CHO) eukaryotic system.
Results: Alpha and beta subunits of LH hormone were cloned between Nhe I and BamH I cut sites of pEGFP_N1 expression plasmid and confirmed by PCR. Hormone expression was evaluated in CHO cell line by Western blotting using the specific antibody.
Conclusion: Alpha and beta subunits of LH hormone were expressed in CHO cell line perfectly.

 
Keyword(s): LH, AMENORRHEA, SPERM, CLONING, RECOMBINANT HORMONE
 
 
References: 
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Click to Cite.
APA: Copy

SOLEIMANIFAR, Z., & BANDEHPOUR, M., & KAZEMI, B. (2016). CLONING AND EXPRESSION OF LUTEINIZING HORMONE SUBUNITS IN CHINESE HAMSTER OVARY CELL LINE. NOVELTY IN BIOMEDICINE, 4(4), 142-146. https://www.sid.ir/en/journal/ViewPaper.aspx?id=569425



Vancouver: Copy

SOLEIMANIFAR ZEINAB, BANDEHPOUR MOJGAN, KAZEMI BAHRAM. CLONING AND EXPRESSION OF LUTEINIZING HORMONE SUBUNITS IN CHINESE HAMSTER OVARY CELL LINE. NOVELTY IN BIOMEDICINE. 2016 [cited 2021May13];4(4):142-146. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569425



IEEE: Copy

SOLEIMANIFAR, Z., BANDEHPOUR, M., KAZEMI, B., 2016. CLONING AND EXPRESSION OF LUTEINIZING HORMONE SUBUNITS IN CHINESE HAMSTER OVARY CELL LINE. NOVELTY IN BIOMEDICINE, [online] 4(4), pp.142-146. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569425.



 
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