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Paper Information

Journal:   NOVELTY IN BIOMEDICINE   2016 , Volume 4 , Number 4; Page(s) 153 To 158.
 
Paper: 

DETECTION OF BLANDM, BLADIM, BLAIMP, BLAVIM AND BLACTX-M-15 BETA-LACTAMASE GENES AMONG PSEUDOMONAS AERUGINOSA AND ACINETOBACTER BAUMANNII STRAINS ISOLATED FROM TWO HOSPITALS OF TEHRAN, IRAN

 
 
Author(s):  FARAJZADEH ALAN TAHERE, GOUDARZI HOSSEIN*, HASHEMI ALI, FALLAH FATEMEH, DOUSTDAR FARAHNOOSH, BOSTAN HOJAT
 
* DEPARTMENT OF MICROBIOLOGY, SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Background: In this study, we evaluated the existence of blaNDM, blaDIM, blaIMP, blaVIM, bla CTX-M-15 beta-lactamase genes among Pseudomonas aeruginosa and Acinetobacter baumannii strains isolated from hospitalized patients.
Materials and Methods: From June 2013 to May 2014, thirty-four nonduplicate nonconsecutive isolates of A. baumannii and P. aeruginosa were isolated from blood, respiratory tract, wound, sputum and urine samples of patients from hospitalized in two hospitals in Tehran, Iran. Antibiotic susceptibility test was performed by Kirby-Bauer disc diffusion method according to CLSI guidelines. In this study, the frequency of MBL (metallo-beta-lactamase) producers was evaluated by CDDT (Combined disk diffusion test) and prevalence of blaNDM, blaDIM, blaIMP, blaVIM and blaCTX-M-15 genes were evaluated by PCR and sequencing methods among P. aeruginosa and A. baumannii strains isolated from hospitalized patient of Tehran during 2013 -2014 years.
Results: Of thirty-four non-fermenter isolates, 24 (70.58%) P. aeruginosa and 10 (29.41%) as A. baumannii were isolated and identified. High rate of resistance to common antibiotics were detected specially among A. baumannii isolates that showed 100% resistance to 4 of tested antibiotics. The CDDT results reveal that 4 (16.66%) of the P. aeruginosa isolates and 1 (10%) of the A.baumannii were positive for production of MBLs. The prevalence of bla CTX-M-15 gene among 10 A. baumannii isolates was 4 (40%), and for IMP-1, 2 (20%). The
??????OXA-51 has been investigated and was detected in all A. baumannii isolates. Also the prevalence of bla CTX-M-15 gene among 24 P.aeruginosa isolates was 11 (45.83%), and for IMP-1, 3 (12.5%). Fortunately, ??????NDM, bla VIM, bla DIM gene was not detected in all isolates.
Conclusion: The detection of MBL-producing A. baumannii and P. aeruginosa strains detected in this research is of great concern and highlights the need of infection control measures, including antimicrobial management and prompt detection of beta-lactamase-producing isolates.

 
Keyword(s): PSEUDOMONAS AERUGINOSA, ACINETOBACTER BAUMANNII, BETA-LACTAMASES
 
 
References: 
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Click to Cite.
APA: Copy

FARAJZADEH ALAN, T., & GOUDARZI, H., & HASHEMI, A., & FALLAH, F., & DOUSTDAR, F., & BOSTAN, H. (2016). DETECTION OF BLANDM, BLADIM, BLAIMP, BLAVIM AND BLACTX-M-15 BETA-LACTAMASE GENES AMONG PSEUDOMONAS AERUGINOSA AND ACINETOBACTER BAUMANNII STRAINS ISOLATED FROM TWO HOSPITALS OF TEHRAN, IRAN. NOVELTY IN BIOMEDICINE, 4(4), 153-158. https://www.sid.ir/en/journal/ViewPaper.aspx?id=569411



Vancouver: Copy

FARAJZADEH ALAN TAHERE, GOUDARZI HOSSEIN, HASHEMI ALI, FALLAH FATEMEH, DOUSTDAR FARAHNOOSH, BOSTAN HOJAT. DETECTION OF BLANDM, BLADIM, BLAIMP, BLAVIM AND BLACTX-M-15 BETA-LACTAMASE GENES AMONG PSEUDOMONAS AERUGINOSA AND ACINETOBACTER BAUMANNII STRAINS ISOLATED FROM TWO HOSPITALS OF TEHRAN, IRAN. NOVELTY IN BIOMEDICINE. 2016 [cited 2021May15];4(4):153-158. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569411



IEEE: Copy

FARAJZADEH ALAN, T., GOUDARZI, H., HASHEMI, A., FALLAH, F., DOUSTDAR, F., BOSTAN, H., 2016. DETECTION OF BLANDM, BLADIM, BLAIMP, BLAVIM AND BLACTX-M-15 BETA-LACTAMASE GENES AMONG PSEUDOMONAS AERUGINOSA AND ACINETOBACTER BAUMANNII STRAINS ISOLATED FROM TWO HOSPITALS OF TEHRAN, IRAN. NOVELTY IN BIOMEDICINE, [online] 4(4), pp.153-158. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569411.



 
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