Paper Information

Journal:   PLANT PROTECTION (SCIENTIFIC JOURNAL OF AGRICULTURE)   WINTER 2018 , Volume 40 , Number 4 #M0053; Page(s) 73 To 86.
 
Paper: 

PURIFICATION, ANTISERUM PRODUCTION AND PHYLOGENY OF IRANIAN SUNFLOWER ISOLATE OF BEAN YELLOW MOSAIC VIRUS (BYMV) BASED ON PARTIAL SEQUENCE OF CI REGION OF THE GENOME

 
Author(s):  MORADI M., HOSSEINI A.*, HOSSEINI S.
 
* DEPARTMENT OF PLANT PROTECTION, FACULTY OF AGRICULTURE, VALI-E-ASR UNIVERSITY OF RAFSANJAN, RAFSANJAN, IRAN
 
Abstract: 

Bean yellow mosaic virus (BYMV), a member of genusPotyvirus, causes economic losses to several crop species due to its wide host range. During 2012-2014 growing seasons, a BYMV isolate causing mosaic and leaf distortion was detected in sunflower fields of Isfahan province (Iran), tentatively designated as BYSun. Virions of BYSun were purified using two cycles of differential centrifugation on 20% and 30% sucrose cushions. Yields were 15-20 mg per 100g of infected leaves. SDS-PAGE of purified virus preparations revealed a single protein band with MW of about 33.5 kDa for BYSun. Antiserum against BYSun was prepared by five subcutaneous injections of purified viruses into rabbits at weekly intervals. Indirect ELISA indicated that the immunoglobulin obtained had the best results in dilution 1/2500. A 680 bp DNA fragment, corresponding to the partial CI (cylindrical inclusion) region of the viral genome was amplified using potyvirus-specific degenerate primers (CIRev/CIFor) by RT-PCR. BLAST search showed that the most similar sequence in the databases was that of BYMV-S. Phylogenetic analysis of this part of the genome in different BYMV isolates sequences revealed six distinct groups including General, Lupin, Broad bean, W, Pea and S which correlated with original natural isolation hosts. BYSun and BYMV-S were grouped apart from other isolates, so they were placed within a distinct group called “S”.

 
Keyword(s): BEAN YELLOW MOSAIC VIRUS, BLAST, SUNFLOWER, TAXONOMIC POSITION
 
References: 
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