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Paper Information

Journal:   EXPERIMENTAL ANIMAL BIOLOGY   FALL 2017 , Volume 6 , Number 2 (22) ; Page(s) 9 To 15.
 
Paper: 

TOTAL FLAVONOID CONTENT AND CYTOTOXICITY OF JURINEA LEPTOLOBA EXTRACT COLLECTED FROM SOUTH OF SHIRAZ ON HUMAN LYMPHOCYTES AND TUMOR HELA CELLS

 
 
Author(s):  TAHERKHANI MAHBOUBEH*
 
* DEPARTMENT OF CHEMISTRY, FACULTY OF SCIENCE, TAKESTAN BRANCH, ISLAMIC AZAD UNIVERSITY, TAKESTAN, IRAN
 
Abstract: 

Cervical cancer is the second most common cancer in women in the world. So far, many natural products have been identified as potent anti-cancer agents. The large Eurasion genusJurinea (tribe Cynareae, subtribe Carduinae) has 250 species. In this study, the aerial parts of Jurinea leptoloba DC., from Compositae were collected from south of Shiraz in August 2012. Defatted extract of J. leptoloba was investigated for total flavonoid content and cytotoxic properties. Total flavonoid content was determined as mg catechin equivalents (CE) / g dried extract using spectrophotometer methods. Total flavonoid content (TFC) of the extract of J. leptoloba was determined to be 98.81±7.74 mg catechin equivalent/g sample. Cytotoxicity was measured using a modified MTT assay on normal human lymphocytes and tumor HeLa cells. The results indicated that the extract of J. leptoloba exhibited a dose-dependent cytotoxicity against HeLa and lymphocyte cells. The LD50 values for HeLa and lymphocyte cells were obtained to be 11.12 mg/ml and 6068.64 mg/ml, respectively The LD50 shows that the cytotoxicity of the extract of J. leptoloba on human cancer cell lines is much higher than that observed in normal lymphocytes. The results obtained suggest that J. leptoloba extract may be exploited as a natural anti-oxidant and anti-cancer agent with low adverse side effects.

 
Keyword(s): CYTOTOXICITY, JURINEA LEPTOLOBA, HELA AND LYMPHOCYTE CELLS, TOTAL FLAVONOID CONTENT
 
 
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Click to Cite.
APA: Copy

TAHERKHANI, M. (2017). TOTAL FLAVONOID CONTENT AND CYTOTOXICITY OF JURINEA LEPTOLOBA EXTRACT COLLECTED FROM SOUTH OF SHIRAZ ON HUMAN LYMPHOCYTES AND TUMOR HELA CELLS. EXPERIMENTAL ANIMAL BIOLOGY, 6(2 (22) ), 9-15. https://www.sid.ir/en/journal/ViewPaper.aspx?id=569115



Vancouver: Copy

TAHERKHANI MAHBOUBEH. TOTAL FLAVONOID CONTENT AND CYTOTOXICITY OF JURINEA LEPTOLOBA EXTRACT COLLECTED FROM SOUTH OF SHIRAZ ON HUMAN LYMPHOCYTES AND TUMOR HELA CELLS. EXPERIMENTAL ANIMAL BIOLOGY. 2017 [cited 2021May15];6(2 (22) ):9-15. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569115



IEEE: Copy

TAHERKHANI, M., 2017. TOTAL FLAVONOID CONTENT AND CYTOTOXICITY OF JURINEA LEPTOLOBA EXTRACT COLLECTED FROM SOUTH OF SHIRAZ ON HUMAN LYMPHOCYTES AND TUMOR HELA CELLS. EXPERIMENTAL ANIMAL BIOLOGY, [online] 6(2 (22) ), pp.9-15. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=569115.



 
 
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