In this experimental study, we investigate the effect of volume fraction and temperature parameters on the relative thermal conductivity of carbon nano- tubes and the new and useful nano- fluid of copper oxide -deionized Water. Nanofluid with volumetric concentrations of 1.0, 2.0, and 6.0 % for evaluation of the effect of the volume fraction on the thermal conductivity was prepared. As well as, to investigate the effect of temperature, the temperature was limited to 30, 40, 50 degrees Celsius. For the measurement of relative thermal conductivity, transient hot wire method is applied by using of hot wire method and probe. With increasing temperature and volume fraction of nano-fluid, thermal conductivity is significantly increased compared to the base fluid. Low volume fractions are significant increase in thermal conductivity. Hence we could obtain thermal conductivity of about 7.38 percent gain that had been left in the field that provides information.