Paper Information

Journal:   KOWSAR MEDICAL JOURNAL   Summer 2004 , Volume 9 , Number Part 2; Page(s) 119 To 126.
 
Paper: 

THE EFFECT OF 20% HEATED AND NON-HEATED FOLLICULAR FLUID ON MATURATION AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES IN VITRO

 
 
Author(s):  IMANI H.*, REZAZADEH M.
 
* 
 
Abstract: 
Objective. In the mice, rate of fertilization and development are important in oocyte matured and a closer imitation of in-vivo conditions during oocyte maturation might be beneficial. The aims of the present study were to investigate whether lmmature mouse oocytes can be matured in-vitro in 20% heated and non-heated human follicular fluid and transferrin and insulin affected follicular fluid on oocyte maturation and development.
Materials and Methods. Immature oocytes were isolated mechanically from 4-6 weeks old NMRI mice ovaries. Oocytes washed and transferred to the drops of medium in following experiment groups for 24 hours: Group I: 207 immature oocytes were cultured in DMEM-Hams F12 containing 20% heated human follicular fluid (hHFF). Group II: 189 immature oocytes were cultured in DMEM-Hams F12 containing 20% Non-heated Human follicular fluid (NhHFF). Group III: 242 immature oocytes were cultured in DMEM-Hams F12 containing 20% hHFF, 5mg/ml Insulin and 10mg/ml transferrin. Group IV: 175 immature oocytes cultured in DMEM-Hams F12 containing 20% NhHFF. 5mg/ml lnsulin and 10mg/ml transferrin.
Results. The overall maturation was significantly higher for oocytes in group III (610/5%) that other groups. The maturation rates were: 610/5% (group III), 41% (group I), 27/4% (group IV) and 10/6% (group II) respectively. The 2-cell formation embryo rates were 56% (group III), 16/7% (group IV). 15/5% (group I) and 5% (group II).
Conclusion. The results demonstrate that (1) mouse immature oocytes can be matured during culture in above medium containing 20% hHFF, NhHFF, Insulin and transferrin (2), maturating and development was higher in presente of 20% hHFF, transferrin and Insulin, (3) the higher regulation of meiotic resumption during in-vitro culture of mouse oocytes might be related to the presence of Insulin and transferrin.
 
Keyword(s): IN VITRO MATURATION, FOLLICULAR FLUID, TRANSFERRIN, INSULIN, IMMATURE OOCYTES, MOUSE
 
References: 
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