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Paper Information

Journal:   KOWSAR MEDICAL JOURNAL   Summer 2004 , Volume 9 , Number Part 2; Page(s) 95 To 100.
 
Paper: 

EFFECT OF THE ACTIVATION OF NITRIC OXIDE SYNTHASE IN THE RAT CENTRAL AMYGDALA ON MORPHINE-INDUCED PLACE CONDITIONING

 
 
Author(s):  KARAMI M.*, SAHRAEI HEDAYAT, ZARINDAST M.R., SEPEHRI H.
 
* 
 
Abstract: 
Objective. Opioids including morphine appear to interact with identical brain reward mechanisms. Morphine-induced place conditioning is a simple learning paradigm. Amygdala has been shown to influence learning processes. The central nucleus of amygdala is also implicated in morphine-induced properties. Nitric oxide (NO) which is formed enzymatically from larginine by NO synthase (NOS), as a neuronal transmitter, is involved in the cellular events that may underlie the processes of learning and memory. In this research the effects of intra-central amygdala injections of NO agents (L-arginine, a prpcursor of NO , and L-NAME, a non-selective inhibitor of NOS) on morphine-induced place conditioning in male Wistar rats have been investigated. Materials and Methods. Animals (255-275g) were implanted with two connulae at the desired coordinates. Eigth animals were used in each experiment. Each animal was conditioned to morphine (0. 0/5-7.5 mg/kg) using a three-day schedule of an unbiased conditioning design. To survey the effects of NO agents on morphine-induced place conditioning, the agents at 0.3-3 ,mg/rat were injected directly into the central amygdala both repeatedly (once per day, during conditioning, prior to morphine injections) and once on the day of testing.
Results. Results showed that only morphine induced a signifiant place conditioning in experimented animals. The maximum response was induced by 5 mgikg of the Qpioid. Administration of morphine (5 mgikg) together with L-arginine both during conditioning and prior to testing of morphine-induced place conditioning increased both the acquisition and expression of the response to morphine. These responses were blocked when L-NAME (0/3-3 ,mg/rat) was administered prior to the injection of the effective dose of L-arginine, while L-NAME by itself showed no significant effect on the response to morphine in these processes.
Conclusion. These results show that NO system in the central nucleus of the rat amygdala has an influence on morphine-induced p!ace conditioning.
 
Keyword(s): MORPHINE, PLACE CONDITIONING, CENTRAL AMYGDALA,NITRIC OXIDE (NO), RAT
 
References: 
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